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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
CUT&Tag-IT™ R-loop Assay Kit
- 保质期:
半年以上
- 供应商:
Active Motif
- 保存条件:
对应组分对应不同保存温度
- 规格:
16 rxns
CUT&Tag-IT® R-loop试剂盒
R-loops是一种特殊的三链核酸结构,由DNA-RNA杂交体和未配对的DNA单链组成。这种loop结构多发生在端粒、着丝粒周围DNA或者转录终止区域。R-loops是基因组的不稳定性的重要来源,和癌症等多种人类疾病相关。Active Motif基于CUT&Tag的方法专门优化实验方案,使得在全基因组范围内分析 R-loop更加轻松简单!
实验速度更快:比 DNA:RNA 免疫沉淀 (DRIP) 方法和染色质免疫沉淀 (ChIP) 方法更快、更高效
信号质量更高:与其他染色质分析技术相比,具有更低的背景和更高的信噪比
兼容范围更广:兼容 200,000 至 500,000 个新鲜或冻存细胞
CUT&Tag-IT® R-loop Assay Kit Contents
The CUT&Tag-IT® R-loop Assay Kit components are shipped at two temperatures, with one box on dry ice for components to be stored at -20°C, and a second box at room temperature for components that are not to be frozen and must be stored at 4°C. Please store components according to the storage conditions in the manual after first use. All reagents are guaranteed stable for 6 months from date of receipt when stored properly.
Reagents included:
- 5% Digitonin, store at -20°C
- R-loop RNase A, store at -20°C
- Protease Inhibitor Cocktail, store at -20°C
- CUT&Tag-IT® Assembled pA-Tn5 Transposomes, store at -20°C
- 10 µg/µL Proteinase K, store at -20°C
- DNA-RNA Hybrid mAb (Clone S9.6), 1 mg/mL, store at -20°C
- Rabbit Anti-Mouse Antibody, store at -20°C
- Tagmentation Buffer, store at -20°C
- Q5 Polymerase Master Mix, store at -20°C
- i7 Indexed Primer 1, store at -20°C
- i7 Indexed Primer 2, store at -20°C
- i7 Indexed Primer 3, store at -20°C
- i7 Indexed Primer 4, store at -20°C
- i5 Indexed Primer 1, store at -20°C
- i5 Indexed Primer 2, store at -20°C
- i5 Indexed Primer 3, store at -20°C
- i5 Indexed Primer 4, store at -20°C
- 1X Binding Buffer, store at 4°C
- Dig-Wash Buffer, store at 4°C
- Concanavalin A Beads, store at 4°C
- SPRI Beads, store at 4°C
- Dig-300 Buffer, store at 4°C
- Antibody Buffer, store at 4°C
- 1% IGEPAL, store at RT
- 0.5 M EDTA, store at RT
- 10% SDS, store at RT
- DNA Purification Wash Buffer, store at RT
- DNA Purification Elution Buffer, store at RT
- DNA Purification Binding Buffer, store at RT
- 3 M Sodium Acetate, store at RT
- DNA Purification Columns SF, store at RT
Active Motif公司是一家致力于研发和生产核细分子生物学和表观遗传学研究用试剂的专业公司,也是世界著名的转录因子试剂生产商和CHIP-seq外包服务供应商。
Active Motif的产品涉及:表观遗传学相关抗体、Chip级别抗体、重组识别子和催化酶、预甲基化修饰的重组组蛋白、多聚核小体和生物素标记核小体;以及各类染色质分析、转录因子表达调控分析和DNA甲基化分析的试剂盒等。
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文献和实验-Seq 的数据表现。该课题组首先对 CUT&Tag 和 ChIP 组进行了相关分析(见图2),发现 CUT&Tag 与 IgG 对照的相关性非常低(r = 0.01),而 ChIP-Seq 组与 mock 对照组的相关性高(r=0.89),表明 CUT&Tag 数据信噪比高。同时研究人员进行了 CUT&Tag 样本重复相关性分析,发现 CUT&Tag 样本之间的重复性非常高(r=0.97)。 图 2. CUT&Tag 与 ChIP-Seq 相关
, T. D., Henikoff, J. G., ... & Henikoff, S. (2019). CUT&Tag for efficient epigenomic profiling of small samples and single cells. Nature communications, 10(1), 1930. [5] Wang, Q., Xioong, H., Ai, S., Yu, X., Liu, Y., Zhang, J., & He, A. (2019
1, For cells cultured in 2D, about 1-2X107 S1 cells were growth in 100mm dish, and were cross-linked by adding formaldehyde to final concentration of 1% and incubated in room temperature for 10 minutes. For cells growth in 3D, cells
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