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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80℃
- 保质期:
90天
- 英文名:
pCDNA3.1-6×His-Ubiquitin-wt
- 库存:
100
- 供应商:
科淼生物
- 规格:
0.5ug/0.5ug
| 规格: | 0.5ug | 产品价格: | ¥680.0 |
|---|---|---|---|
| 规格: | 0.5ug | 产品价格: | ¥1280.0 |
英文名称:pCDNA3.1-6×His-Ubiquitin-wt
货号:KM1030476
图谱:微客服
宿主:哺乳细胞
用途:蛋白表达
片段类型:ORF
片段物种:人
原核抗性:Amp
真核抗性:Neo/G418
荧光标记:
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文献和实验-MEM、DMEM培养基和M-MLV逆转录酶;小牛血清;G418;DIG High PrimeDNA Labeling and Detection Starter Kit I1。 质粒 pGL3-promoter;含EBVR的质粒p220.2。 重组质粒载体pcDNA3-EGFP-EBVR的构建 采用StuI和XbaI消化p220.2,获得长4.7 kb的EBVR,包括EBV核抗原1(EBNA1)和OriP,克隆至pGL3-promotor的SV40启动子下游Hind Ill
玉米Proline responding 1(pro1)突变在蛋白合成和细胞周期调控中起到关键作用
in1/2 MS culture medium supplemented with various concentrations of L-proline. Ubiquitin was used as an internal control. For each sample, three technical and two independent biological replicates were performed. Error bars represent SD.wt, wild type
can be selectively inhibited by targeting the catalytic site with drug?like compounds. Notably, the mammalian 26S proteasome is associated with three major DUBs: RPN11, UCH37, and USP14. Because the ubiquitin ?chain?trimming? activity of USP14 can inhibit proteasome
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