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- 技术资料
- 保存条件:
-80℃
- 保质期:
90天
- 英文名:
pEnCMV-6×His-TOMM70(human)
- 库存:
100
- 供应商:
科淼生物
- 规格:
0.5ug/0.5ug
| 规格: | 0.5ug | 产品价格: | ¥680.0 |
|---|---|---|---|
| 规格: | 0.5ug | 产品价格: | ¥1280.0 |
英文名称:pEnCMV-6×His-TOMM70(human)
货号:KM1032977
图谱:微客服
宿主:哺乳细胞
用途:蛋白表达
片段类型:ORF
片段物种:人
原核抗性:Amp
真核抗性:Neo/G418
荧光标记:
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文献和实验PriCells: Methods of Isolation and Culture of Human Hepatocytes
should be viewed using a standard laboratory 16 binocular microscope (×400 magnification). Typically 70–90% of isolated human hepatocytes exclude trypan blue. Suspensions of isolated human hepatocytes can be used for short-term experiments, i.e., up to 4 h
Flow Cytometry: Immunofluorescence Staining of Activated and Resting Human Platelets
for 30 minutes at room temperature.6. Wash two times in PBS and resuspend cell pellet in PBS. Cells may be stored at 4℃ for up to 8 hours.7. Stain activated platelets by direct immunofluorescence using CD62P-FITC, catalog number 555523 (31794×), at 20 μL
PriCells: Introduction of Isolation and Culture of Human Primary Hepatocytes
perfusion method for preparing hepatocytes appears to be the most suitable method for utilizing the available material. The preparation of human hepatocytes using this method has produced yields of between 0.1–30 × 106 hepatocytes/g wet liver with cell
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