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文献和实验1 hour @ 37℃.Add 2ul of DNase I (Promega) to each transcription, incubate 20 minutes @ 37℃.Part II: Probe PurificationPurify probes using QIAGEN QIAquick Nucleotide Removal Kit or Boehringer Spin Columns (G50 Sephadex).Check 1μl in scintillation counter
and Desjardins, ), RNA purification by purification kit (Phenol‐Free Total RNA Purification Kit or Ribozol Plus RNA Purification Kit; AMRESCO Inc., http://www.amresco‐inc.com/), lithium chloride precipitation (Diaz‐Ruiz and Kaper, ), spin‐column
in your own lab to detect RNases and a protocol is provided in Technical Bulletin 166 to facilitate this. However this assay is time consuming and requires working with radioactivity. As an alternative, Ambion's RNaseAlert™ Kit (Cat #1964) allows researchers
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