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- 2025年10月05日
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文献和实验MitoProbe DiIC1 Mitochondrial Membrane Potential Protocol
can be added simultaneously with DiIC1(5). Titration of the CCCP may be required for optimal results with each cell type. 3) Add 5 μL of 10 μM DiIC1(5) (supplied with the kit, 50 nM final concentration) and incubate the cells at 37°C, 5% CO2
Relation Between Mitochondrial Membrane Potential and ROS Formation
to harmless H2 O. In this chapter, we describe a relation between mitochondrial membrane potential and the rate of ROS formation. We present different methods applicable for isolated mitochondria or intact cells. We also present experiments demonstrating
Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured Cells
The mitochondrial membrane potential is the dominant component of the proton-motive force that is the potential term in the proton circuit linking electron transport to ATP synthesis and other energy-dependent mitochondrial processes
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