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- 2025年09月27日
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文献和实验CellTrace CFSE Cell Proliferation Kit
in phosphate-buffered saline (PBS) or other suitable buffer to the desired working concentration (0.5–25 μM). Resuspend the cells gently in prewarmed (37°C) PBS containing the probe (prepared in previous step). Incubate the cells for 15 min
reverse genetics allow straightforward labeling of yeast proteins in living cells. Budding and fission yeast are therefore attractive organisms in which to study dynamic cellular processes such as growth, cell division, and morphogenesis using live cell
Live Cell Fluorescence Microscopy Techniques
The use of fluorescent tags for in vivo tracking of proteins has provided an array of new data on cell function. Correspondingly, a variety of new methods utilizing these fluorescent tags have been developed. These methods
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