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文献和实验Cloning Polymerase Chain Reaction Products Utilizing theT/A Overhang and a Kit
to the ligation reaction mixture. This procedure is based on the fact that Taq polymerase has a nontemplate dependent activity that adds a single deoxyadenosine to the 3′ ends of double-stranded DNA (2 ). The Invitrogen pCR™II vector is supplied as a linearized
LIVE/DEAD® Violet Viability/Vitality Kit
of labelings: At the recommended reagent concentrations and volumes, this kit contains sufficient material to perform approximately 200 assays using flow cytometry. Approximate fluorescence excitation/emission maxima: calcein violet 400/452 nm; aqua
Vybrant® DyeCycle Green and Orange Stains
material is supplied for approximately 200 fl ow cytometry assays based on a 1 mL test volume. Approximate fluorescence excitation/emission maxima: Vybrant® DyeCycle™ Green stain: 506/534 nm, bound to DNA; Vybrant® DyeCycle™ Orange stain: 519/563 nm
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