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文献和实验How short can be the CpG island-Molecular Biology
the criteria to 500 bp. QUOTE is that possible the short CpG sequence be methylated? It may be methylated. If you are going to map this area, be cautioned that the accepted concept is, in normal cells, CpG sites outside CpG islands are usually methylated
CpG Island length variation-DNA Methylation, Histone and
----------------------- Hi Chris, Based on Gardiner-Garden's criteria, CpG islands are generally 500 bp in length [1]. Later, Jones et al [2] modified the criteria and suggested that regions of DNA of greater than 500 bp with a G+C equal to or greater than 55% and observed
Methylated CpG Island Amplification
for up to 6 months. Mix the following: 500 ng of Digested DNA , 10 m l of adaptor mixture, 400 Units of T4 DNA ligase, 3 m l of 10X ligase buffer and water to a total volume of 30 m L. Incubate at 16℃ for 3-16 hours. 2.1.3 PCR Amplification
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