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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
CD335 (NKp46)
- 级别:
CD335 (NKp46) Antibody
- 目录编号:
CD335 (NKp46) Antibody
- 克隆性:
单克隆
- 抗原来源:
大鼠
- 保质期:
CD335 (NKp46) Antibody
- 抗体英文名:
Fire™ 750 anti-mouse CD335 (NKp46) Antibody-APC
- 抗体名:
CD335 (NKp46) Antibody
- 标记物:
APC
- 宿主:
大鼠
- 适应物种:
小鼠
- 免疫原:
CD335 (NKp46) Antibody
- 亚型:
CD335 (NKp46) Antibody
- 形态:
CD335 (NKp46) Antibody
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
0.2 mg/mL
- 规格:
25 µg/100 µg
| 规格: | 25 µg | 产品价格: | ¥2500.0 |
|---|---|---|---|
| 规格: | 100 µg | 产品价格: | ¥5900.0 |
Description :CD335, also known as NKp46, is a single-pass type I membrane protein of 46 kD. It belongs tothe natural cytotoxicity receptor (NCR) family and contains two Ig-like (immunoglobulin-like)domains. It's expression is restricted to NK cells and a subset of NKT cells; it's not expressed inCD1d-restricted NKT cells. CD335 is a receptor for viral hemagglutinins and heparan sulfateproteoglycans and is involved in NK cell activation.
Verified Reactivity :Mouse
Antibody Type :Monoclonal
Host Species :Rat
Immunogen :NKP46-IgG1 Fc fusion protein
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation :The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 underoptimal conditions.
Concentration :0.2 mg/mL
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cellsin 100 µl volume. It is recommended that the reagent be titrated for optimal performance for eachapplication.
* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.
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文献和实验1. Walzer T, et al. 2007. P. Natl. Acad. Sci. USA 104:3384. (FC, Activ)
2. Walzer T, et al. 2007. Nat. Immunol. 8:1337. (FC, Activ)
3. Guerriero JL, et al. 2011. J. Immunol. 186:3517. (IHC) PubMed
Product Citations:
1. Sweeney EE, et al. 2020. Nano Res. 13:736. PubMed
2. Li D, et al. 2020. Immunohorizons. 0.661805556. PubMed
3. Dubrot J, et al. 2021. Immunity. 54(3):571-585.e6. PubMed
抗体二抗上连接有荧光染料。直接标记染色背景低,实验程序少尽量减少实验工序和过程,以保证实验的真实和准确性。因此在条件允许的范围内,建议尽量用直接标记的抗体进行实验。 3、流式抗体荧光标记的选择: 如果实验中检测单一指标:不同荧光标记在不同的仪器上强度不同。以某仪器为例:PE >APC >PE-Cy5 >PerCP >FITC >PerCP-Cy5.5,通常来说,PE最强,适用于弱表达抗原。FITC强度较弱,适用于强表达抗原,使用范围比较广。用户需根据检测的目标蛋白进行具体选择。 如果同时检测多个
γRⅢ/Ⅱ 结合,封闭非特异性染色,使阴性细胞的背景荧光降至未标记细胞的水平。加入 0.5-1 μg 纯的抗小鼠 CD16/32 单克隆抗体,室温孵育 10 分钟。 对于人和大鼠,可直接使用过量的,与荧光抗体相同来源和亚型的纯化 Ig 或者与目标种属相同来源的血清进行阻断,或者用商业化的 Fc 受体阻断剂。 五、细胞染色 按照说明书的推荐用量加入荧光标记抗体,混匀后置于 4℃,避光孵育 30 分钟。 加入细胞染色 buffer (或含 1%BSA 的 PBS)重悬细胞,300g 离心
而导致荧光亮度有限。基于Biotium科学家研制的新型分子工程技术,近红外CF 染料克服了这些问题。即使在633 nm 被激发,CF750 也会比APC-AlexaFluor 750拥有足够的亮度来在流式细胞仪检测中带来更好的信噪比,而且不存在串联染料的低稳定性等问题。另外,近红外CF 染料高度水溶性,没有会增加抗体耦联物非特异性结合的过剩的负电荷,也代表了该产品的优秀性能。











