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5x1 ml
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文献和实验Vybrant® DyeCycle Green and Orange Stains
material is supplied for approximately 200 fl ow cytometry assays based on a 1 mL test volume. Approximate fluorescence excitation/emission maxima: Vybrant® DyeCycle™ Green stain: 506/534 nm, bound to DNA; Vybrant® DyeCycle™ Orange stain: 519/563 nm
in the bottom of the well. Materials DNA samples DNA size standard Loading dye solution Agarose TBE buffer Sybr Green I solution diluted 10,000-fold Dark Reader light box Plastic wrap
onto a piece of plastic wrap (one for each DNA sample)Pipette 5 ul of each DNA sample into one of the drops of loading dye. Mix by pipetting up and down and carefully load into a well of the agarose gel.Pipette 10 ul of a solution containing a DNA size standard
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