Sunrise 货号4707-300 2X ACCM-D(-

2X ACCM-D (-Glucose-Pro) Powder, 10 x 0.5 liter pouches
CATALOG #: 4707-300

Recipe in mg/L:
SALTS, VITAMINS, MINERALS & TRACE ELEMENTS
Calcium chloride dihydrate (26.4 mg/L)
Citric acid (5136 mg/L)
Citric acid trisodium dihydrate (9480 mg/L)
Iron (II) sulfate heptahydrate (5.6 mg/L)
Magnesium chloride hexahydrate (400 mg/L)
Potassium phosphate monobasic anhydrous (1000 mg/L)
Sodium chloride (14560 mg/L)
AMINO ACIDS & SUPPLEMENTS L-Alanine (224 mg/L)
L-Arginine (316 mg/L) L-Asparagine monohydrate (176 mg/L)
L-Aspartic acid (144 mg/L)
L-Cysteine hydrochloride monohydrate (548 mg/L)
L-Glutamic acid potassium salt monohydrate (1344 mg/L)
L-Glutamine (712 mg/L)
L-Glycine (176 mg/L)
L-Histidine hydrochloride monohydrate (110 mg/L)
L-Isoleucine (224 mg/L) L-Leucine (462 mg/L)
L-Lysine hydrochloride (522 mg/L)
L-Methionine (136 mg/L)
L-Phenylalanine (208 mg/L)
L-Serine (354 mg/L)
L-Threonine (244 mg/L)
L-Tryptophan (100 mg/L)
L-Tyrosine (218 mg/L)
L-Valine (322 mg/L)
OTHER ADDITIVES Methyl-b-cyclodextrin (2000 mg/L)
RPMI 1640-Glutamine-Amino acids-Glucose (2000 mg/L)

Instructions
For a 1X solution, add pouch contents to 1000mL deionized water.
For a 2X solution, add pouch contents to 500mL deionized water.
Adjust pH to 4.75 with 6N NaOH and filter sterilize.

NOTE:
This optimized formulation contains several highly-hydrated ingredients. Some clumping of
powder may be expected and should be easily dispersed when stirred to dissolve. Store powder and
prepared medium at 2C-8C.

Suggested g/L: 41.148
Storage Temperature: 2-8 C

Related Categories
Nucleic Acid & Protein Purification, Lab Tools