- ￥950
- QIAGEN
- 19155
- 德国
- 2025年10月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
QIAGEN Protease
- 保质期：
2年
- 供应商：
科邦兴业北京科技有限公司
- 保存条件：
常温
- 规格：
kit
QIAGEN Protease (7.5 AU)
Cat. No. / ID: 19155
7.5 Anson units per vial (lyophilized)
Features
- For protease digestion in DNA and RNA isolation procedures
- Fully compatible with selected QIAGEN protocols
- Both enzymes quality-guaranteed by QIAGEN
Product Details
QIAGEN Protease and QIAGEN Proteinase K offer broad substrate specificity with high activity for a wide range of reaction conditions. Both proteases offer high activity in buffers commonly used in most DNA and RNA isolation procedures and are quality-guaranteed by QIAGEN.
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文献和实验elution buffer combine supernatants, incubate at 65 °6 h to ON take 1/100 of the protein amount taken for the IP, add to 150 µl elution buffer (INPUT control) incubate at 65 °C 6 h to ON add 750 µl PB buffer (Qiagen
DNA Purification from Blood or Body Fluids
in step 11. ■ Ensure that Buffer AW1, Buffer AW2, and QIAGEN Protease have been prepared according to the instructions on page 17. ■ If a precipitate has formed in Buffer AL, dissolve by incubating at 56°C. Procedure 1. Pipet 20 µl QIAGEN
Buffer G2，最大速漩涡10-30s，充分重悬核酸沉淀物（时间要控制得很严格）。(10)加入25μl QIAGEN Protease，50℃保育30-60min（如有必要可延长保育时间）。2）DNA的提取二、基因组步行法扩增5’及3’侧翼序列1 材料基因组DNA2．仪器、用具 离心机、PCR仪 3．试剂 (1)30μl DraⅠ(10units/μl) (2)10μl T4 DNA Ligase (6units/μl) (3)10×Ligation Buffer (4)BD Genome
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