T4 DNA Polymerase

Using T4 DNA Polymerase to Generate Clonable PCR Products

Polymerase chain reaction (PCR) mediated through Taq DNA polymerase has become a simple and routine method for cloning, sequencing, and analyzing genetic information from very small amounts of materials (1 ). Taq DNA polymerase

Ligation of DNA with T4 DNA Ligase

recombination and DNA synthesis. DNA ligase from E. coli is a polypeptide with a molecular weight of 74,000 and is NAD-dependent. T4 DNA ligase is the product of gene 30 of the T4 phage, has a molecular weight of 68,000, and is ATP-dependent. Both enzymes

什么是T4 DNA连接酶

相关专题 重组DNA技术工具酶 T4 DNA Ligase即T4 DNA连接 酶，可以催化粘端或平端双链DNA或RNA的5’-P末端和3’-OH末端之间以磷酸二酯键结合，该催化反应需ATP作为辅助因子。同时T4 DNA连接酶可以修补双链DNA、双链RNA或DNA/RNA杂合物上的单链缺刻(single-strand nicks)。