- ￥339.90
- Thermo Scientific
- USA
- EP0061
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20
- 保质期：
2年
- 英文名：
thermo
- 库存：
1000
- 供应商：
北诺生命科学
- 规格：
100 units
公司产品线包括：FastDigest & 常规限制性内切酶、修饰酶、PCR & RT-PCR产品、核酸和蛋白质Markers、分子生物学试剂盒（分子克隆、核酸纯化、标记、检测）、转染试剂、核苷酸等。
Unstained Protein MW Marker, 2 x 1 mL. 26610(SM0431) Pierce
Prestained Protein MW Marker, 500 uL. 26612(SM0441) Pierce
PageRuler Unstained Protein Ladder, 2 x 250 uL. 26614(SM0661) Pierce
PageRuler Prestained Protein Ladder, 2 x 250 uL. 26616(SM0671) Pierce
PageRuler Prestained Protein Ladder, 10 x 250 uL. 26617(SM0672) Pierce
PageRuler Plus Prestained Protein Ladder, 2 x 250 uL. 26619(SM1811) Pierce
PageRuler Plus Prestained Protein Ladder, 10 x 250 uL. 26620(SM1812) Pierce
Spectra Multicolor Broad Range Protein Ladder, 10 x 250 uL. 26623(SM1842) Pierce
Spectra Multicolor High Range Protein Ladder, 2 x 250 uL. 26625(SM1851) Pierce
Spectra Multicolor Low Range Protein Ladder, 250 uL. 26628(SM1861) Pierce
PageRuler Broad Range Unstained Protein Ladder, 26630(SM1881) Pierce
PageRuler Low Range Unstained Protein Ladder, 2 x 250 uL. 26632(SM1891) Pierce
Spectra Multicolor Broad Range Protein Ladder, 2 x 250 uL. 26634(SM1841) Pierce
10X Buffer Cfr9I B02 Fermentas
10X Buffer Cfr10I B04 Fermentas
10X Buffer EcoRI B12 Fermentas
10X Buffer Bsp143I B13 Fermentas
Bovine Serum Albumin B14 Fermentas
10X Taq Buffer with KCl and 15 mM MgCl2 B16 Fermentas
Dilution Buffer for Restriction Enzymes B19 Fermentas
10X Buffer Eco52I B22 Fermentas
10X Buffer SduI, Ppu21I B23 Fermentas
10X Buffer SdaI B24 Fermentas
10X Buffer Eam1105I B25 Fermentas
10X Buffer Ecl136II, PacI, SacI B26 Fermentas
10X Buffer AarI, AjiI, Bpu10I, ScaI, PasI B27 Fermentas
10X Buffer TaqI B28 Fermentas
10X Buffer KpnI B29 Fermentas
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文献和实验Using T4 DNA Polymerase to Generate Clonable PCR Products
Polymerase chain reaction (PCR) mediated through Taq DNA polymerase has become a simple and routine method for cloning, sequencing, and analyzing genetic information from very small amounts of materials (1 ). Taq DNA polymerase
Ligation of DNA with T4 DNA Ligase
recombination and DNA synthesis. DNA ligase from E. coli is a polypeptide with a molecular weight of 74,000 and is NAD-dependent. T4 DNA ligase is the product of gene 30 of the T4 phage, has a molecular weight of 68,000, and is ATP-dependent. Both enzymes
相关专题 重组DNA技术工具酶 T4 DNA Ligase即T4 DNA连接 酶，可以催化粘端或平端双链DNA或RNA的5’-P末端和3’-OH末端之间以磷酸二酯键结合，该催化反应需ATP作为辅助因子。同时T4 DNA连接酶可以修补双链DNA、双链RNA或DNA/RNA杂合物上的单链缺刻(single-strand nicks)。
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