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- Fermentas
- EN0191
- 2025年07月16日
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文献和实验Footprinting with Exonuclease III
but also because of an increase in the available probes that can be chosen in order to tackle a specific problem. There are two major groups of probes-the chemical probes and the enzymatic probes. These enzymatic probes, such as DNase I or exonuclease III, have the advantage
The protocol for LIC by Exonuclease III
The protocol for LIC by Exonuclease III 梁耀极 1. Design the primers with 15-bp overlap; 2. Digest the vector by proper restriction enzyme; For getting high quality vectors, there are some good advices as follows: 1) The restriction
Ordered Deletions Using Exonuclease III
in the vector. Deletions are generated by digesting DNA unidirectionally with Escherichia coli exonuclease III (ExoIII) (1 ). ExoIII digests one strand of double-stranded DNA by removing nucleotides from 3′ ends if the end is blunt or has a 5′ protrusion. A 3′
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