Description :Helios is a member of the Ikaros family of zinc finger transcription factors. It contains a Cterminal region composed of 2 zinc-finger domains that mediate dimerization between thefamily members. Helios was originally cloned from a mouse thymoma line. It is mainlyexpressed in peripheral T cells and thymocytes. It is found at high levels in a subpopulation ofregulatory T cells. Helios plays an important role in T cell development and homeostasis.Overexpression of Helios profoundly alters αβ T cell differentiation and activation. It has beendetermined that silencing of Helios in B cells is critical for maintaining normal B cell function.Helios is also involved in tumor immunity.
Verified Reactivity :Mouse, Human
Antibody Type :Monoclonal
Host Species :Armenian Hamster
Immunogen :Helios peptide (aa 51-107)
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation :The antibody was purified by affinity chromatography, and conjugated with APC under optimalconditions.
Concentration :Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :ICFC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by intracellular flow cytometry using our TrueNuclear™ Transcription Factor Staining Protocol.For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µlstaining volume or 5 µl per 100 µl of whole blood.It is recommended that the reagent be titrated foroptimal performance for each application.
Application References(PubMed link indicatesBioLegend citation):
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2. Verhagen J and Wraith D. 2010. J. Immunol. 185:7129.
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6. Bedke T, et al. 2014. Immunol Cell Biol. PubMed
7. Liu Y, et al. 2014. Am J Physiol Gastrointest Liver Physiol. 307:177. PubMed
8. Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (FC) PubMed
Product Citations:
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12. Yang YH, et al. 2020. Cell Reports. 30(5):1515-1529.e4. PubMed