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50
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AbMole
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5mg
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文献和实验Two dimensional peptide mapping
This specfic protocol is the latest incarnation of peptide mapping procedures that have been developed here in the TVL/MBVL of the Salk Institute over the last 20 years. Wade Gibson developed the first peptide mapping protocol
Strep/FLAG Tandem Affinity Purification (SF‐TAP) to Study Protein Interactions
followed by elution with desthiobiotin. (2) Purification by the FLAG‐tag moiety: binding to anti‐FLAG M2 affinity matrix followed by elution with FLAG peptide. Abbreviations: sp., specific interactors (shown as gray circles); n.sp., nonspecific proteins
,with the development of ESI-MS and MALDI-MS.Peptide mass fingerprinting uses the peptide masses obtained by digestion to search the protein and DNA databases,to find proteins that show a similar theoretical digest pattern (see eg .,Shevchenko et al.1996a and b
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