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- 库存:
50
- 供应商:
AbMole
- CAS号:
1070790-89-4
- 规格:
5mg
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文献和实验Yeasts as a Tool for Heterologous Gene Expression
and the viral HBsAg in P. pastoris and S. cerevisiae using the strong and inducible promoters AOX1 and Gal10/Cyc1, respectively. Besides the production as a goal of both expressions, we also report on an unexpected result that has occurred in S. cerevisiae
Setting Up Reverse Transcription Quantitative-PCR Experiments
dehydrogenase (GAPDH ), cyclophilin (CYC ), and elongation factor 1α (EF-1α )] frequently used housekeeping genes in rice. The expression stability of the five selected housekeeping genes varied considerably in different tissues (seedlings, vegetative
/Cytoperm液、Perm/Wash液和FACS Lysing Solution,均购自BD Pharmingen。(4)抗体细胞表面标记抗体:抗人CD3-APC,CD8-PercP,CD8-CYC,CD4-CYC。抗小鼠CD4-CYC、CD8PE。细胞内因子抗体:抗人IL-4-PE,IFN-γ-FITC。抗鼠IL-4-PE,IFN-γ-FITC。以上抗体均购自BD Pharmingen。2、实验方法(1)外周血单个核细胞的分离:取肝素抗凝正常人或者Balb/c小鼠外周血1.5ml。在试管内加入淋巴
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