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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
AbMole
- CAS号:
13368-13-3
- 规格:
5g
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文献和实验to 7.0 Distilled water 100 ml Store at room temperature 0.5 M EGTA Stock* 19.02 g EGTA (Sodium Salt) distilled water to 90 ml Adjust pH to 7.0 Distilled water to 100 ml Store at room temperature 1M dithiothreitol (DTT)* 1.542 g
+ 0.01% Triton X-100 + 1mM DTT + 1mM EGTA. Variables include lowering salt (above recipe is about 136mM in NaCl). replacing EGTA with 1m M CaCl2, replacing ATP with ADP. ATP Sigma#A5394 ADP Sigma #A6521 10X PBS: 80 grams NaCl + 2 grams
+ 0.1% BSA + 0.01% Triton X-100 + 1mM DTT + 1mM EGTA. Variables include lowering salt (above recipe is about 136mM in NaCl). replacing EGTA with 1m M CaCl2, replacing ATP with ADP. ATP Sigma#A5394 ADP Sigma #A6521 10X PBS: 80 grams NaCl
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