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- 库存:
50
- 供应商:
AbMole
- 规格:
1mLA+1mLB
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文献和实验Purification of Inhibitor-Free Soil DNA Obtained by other Methods
实验步骤 1. Dissolve the DNA pellet with 200 ul of Elution Buffer or Buffer TE. Important: DNA pellet should be dissolved in Elution Buffer or Buffer TE(pH8.0). 2. Add 50 ul inhibitor Removal Resin to the sample
ALKALINE PHOSPHATASE (APAAP) TECHNIQUE
. DO NOT DIFFERENTIATE IN ACID ALCOHOL. 26. Mount in an AQUEOUS MOUNTANT. Results --Nuclei Blue --Sites of alkaline phosphatase activity Red Substrate Solution Naphthol-AS-MX phosphatase, free acid (Sigma N 4875) 2 mg N,N-Dimethyl formamide
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
-Phos staining. Background staining can be due to endogenous alkaline phosphatase activities or reduction of NBT from other sources (e.g. NADPH). It is also enhanced by light. The most effective inhibitor is heat. To reduce background
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