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文献和实验Expression of Recombinant Antibodies in Mammalian Cell Lines
Since the advent of hybridoma technology, mammalian-cell culture has been employed for the expression and high-level production of monoclonal antibodies (MAbs). Recent adaptations in recombinant technology have developed the use of numerous
Expression of Recombinant MMP-28 in Mammalian Cells
The expression of a recombinant MMP in a mammalian cell line can be useful, e.g., for purification of the enzyme, to characterize function of the enzyme, or to uncover its substrates. In this chapter, we have therefore documented
Recombinant DNA Engineering, Or Cloning Genes In Plasmids
have a promoter upstream of the MCS such that the insert gets transcribed. If the promoter is mammalian (e.g., the CMV promoter), the transcription will take place only in mammalian systems (like mammalian cell culture), but not in the bacteria. Plasmids
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