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50
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AbMole
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10mg
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文献和实验Expanding Human T Regulatory Cells with the mTOR-Inhibitor Rapamycin
increasing momentum. However, given the limited amount of circulating FOXP3+ Treg cells, efficient methods for their ex vivo expansion are highly desirable. Rapamycin allows for in vitro expansion of murine and human FOXP3+ Treg cells, which maintain
-amylases of diverse origin. Three major groups of α-amylase have been characterized, based on molecular weight: 60,000, 24,000, and 12,500 Da. The inhibitor forms a complex with amylase. The complex formation can inactivate the amylase and in turn cause
for cell lysis: 10 mM Tris-HCl, pH 7.4,50 mM NaCl, 5 mM EDTA, 50 mM NaF, 30 mM Na 4 P 2 O 7 ,and 1% (v/v) Triton-X100. 5. Protease inhibitors and phosphatase inhibitor are added fresheach time with the following final concentrations: 10 μg/mLaprotinin
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