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- Abmole
- AbMole
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- 2025年07月09日
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文献和实验range of recombinant proteins. Here, we describe the methodology associated with expressing a variety of molecular chaperones in Chinese hamster ovary (CHO) lines in order to improve their recombinant protein production capacity. These chaperones include
Generation of High-Recombinant-Protein-Producing Chinese Hamster Ovary (CHO) Cells
The purpose of this chapter is to propose a practical procedure for the generation and selection of Chinese hamster ovary cells producing high levels of recombinant protein by combining in vitro and in vivo amplification of the foreign gene
up 300 ml of CHO medium to 37 deg.C and add cytochalasin B (150 ul of 10 mg/ml) and nocodazole (300 ul of 10 mg/ml) Make sure all buffers are in coldroom, there is rocker in the coldroom and there is a good aspirator in the coldroom. Hook up a sawed
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