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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
AbMole
- CAS号:
600734-06-3
- 规格:
2mg
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文献和实验用GC、GC/MS方法检测人尿中Selegiline及其代谢物
摘要:本文采用GC、GC/MS技术对口服Selegiline Hydrochloride,一种单胺氧化酶抑制剂的尿中代谢物进行分析测定,确认检出10种代谢产物,其中三种为新检测到的羟基化代谢物。 1.尿样收集 健康男性(体重65 Kg)口服单次剂量10mg Selegiline 5片剂。分别收集所排各次尿液的全部及服药前的空白尿,于-20℃。 2.样品制备 取尿样5 ml,加入100 mg L-半胱氨酸,0.5 ml浓HCl
earth. After RNase treatment, the DNA containing supernatant is bound to diatomaceous earth in a chaotropic buffer of guanidine hydrochloride by incubation at room temperature. The DNA-associated diatomaceous earth then is collected by centrifugation
Chemical Co. (St. Louis, Mo, USA). BAY 11-7082 (BAY) was from Calbiochem (La Jolla, Calif, USA). RAW264.7 and U937 cells were purchased from ATCC (Rockville, Md, USA). All other chemicals were of reagent grade. Aggregation-inducing antibody against CD
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