- ￥1400
- Abmole
- AbMole
- M11478-500g
- 2025年11月18日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
AbMole
- CAS号：
34482-63-8
- 规格：
500g
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文献和实验acetate 2.0 M Sucrose with 0.0033 M calcium acetate 0.075 M NaCl with 0.024 M EDTA, pH 8.0 Tris-HCl buffer, pH 8.0 with the following molarities 0.05 M, 0.002 M, 0.0004 M TCA (Trichloroacetic acid
This is a very fast mini-prep protocol which is suitable for sequence analysis and restriction digests. Although the yield is higher than Protocol D.1, there is considerable chromosomal DNA, RNA and protein contamination. Solutions Sucrose/Tris
Preparation of Brain Membrane Fractions
pipette.Leave the homogenate on ice for 45 min to lyse synaptosomes and vesicles.Homogenize again with 10 strokes of the piston.Supplement the homogenate to get a final concentration of 34% (w/w) sucrose and 5 mM Tris-acetate, pH 7.4.Use this homogenate
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