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文献和实验-8878.3. Takayama, H. et al. 2008. High-level expression, single-step immunoaffinity purification and characterization of human tetraspanin membrane protein CD81. PLoS ONE 3: e2314 (DOI: 10.1371/journal.pone.0002314).4. Zhong, M. and Molday, R.S. 2010
v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性
and HEK293 cells weretreated for 3 h either with conditioned medium from parental L cells ( L ) or Wnt-3a/L celltransfectants. Varying concentrations of sFRP-1 had been added to the Wnt-3a medium30 min before incubation with cells. Cell extracts
secondary antibody review -- data from 99 publications
IgG Alexa Fluor 488 1:250 Molecular Probes 10 goat Cy3 immunocytochemistry detect NMDA receptors in HEK293 cells Jackson ImmunoResearch Laboratories 10 FITC immunocytochemistry 1:100 detect phospho-CREB in human colonic
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