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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
P2X7R
- 级别:
P2X7R Antibody
- 目录编号:
P2X7R Antibody
- 克隆性:
单克隆
- 抗原来源:
大鼠
- 保质期:
P2X7R Antibody
- 抗体英文名:
TotalSeq™-A0824 anti-mouse P2X7R Antibody
- 抗体名:
P2X7R Antibody
- 标记物:
P2X7R Antibody
- 宿主:
大鼠
- 适应物种:
小鼠
- 免疫原:
P2X7R Antibody
- 亚型:
P2X7R Antibody
- 形态:
P2X7R Antibody
- 应用范围:
PG
- 保存条件:
2-8℃
- 浓度:
0.5 mg/ml
- 规格:
10 ug
Description :P2X7R, also known as P2X7 receptor, belongs to the family of ligand-gated ion channelreceptors. It is expressed on T cells, B cells, macrophages, and microglia. The receptor opensin the presence of extracellular ATP or NAD, leading to intracellular calcium mobilization.P2X7R activation requires higher concentrations of ATP compared to other P2X receptors.Longer stimulation results in larger pores, allowing passage of larger molecules. Activation ofthese molecules also leads to mitochondrial and cytoskeletal changes as well as IL-1βmaturation and release. Ligation of P2X7 receptor can lead to membrane blebbing and celldeath.
Verified Reactivity :Mouse
Antibody Type :Monoclonal
Host Species :Rat
Immunogen :Murine colon mast cells
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA.
Preparation :The antibody was purified by chromatography and conjugated with TotalSeq™-A oligomer underoptimal conditions.
Concentration :0.5 mg/ml
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application :PG - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-A antibodies arecompatible with 10x Genomics Single Cell Gene Expression Solutions.
To maximize performance, it is strongly recommended that the reagent be titrated for eachapplication, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2- 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to thecell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent fortitration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™protocol for specific staining instructions.
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文献和实验1. Kurashima Y, et al. 2012. Nat. Commun. 3:1034. (FC, IP, WB, IHC, FA)
Product Citations:
1. Hoppe A, et al. 2020. J Allergy Clin Immunol. 1476:145. PubMed
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