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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
CD42d
- 级别:
CD42d Antibody
- 目录编号:
CD42d Antibody
- 克隆性:
单克隆
- 抗原来源:
仓鼠
- 保质期:
CD42d Antibody
- 抗体英文名:
Purified anti-mouse/rat CD42d Antibody
- 抗体名:
CD42d Antibody
- 标记物:
CD42d Antibody
- 宿主:
仓鼠
- 适应物种:
小鼠、大鼠
- 免疫原:
CD42d Antibody
- 亚型:
CD42d Antibody
- 形态:
CD42d Antibody
- 应用范围:
FC, IP
- 保存条件:
2-8℃
- 浓度:
0.5 mg/ml
- 规格:
50 µg
Description :CD42d is an 83 kD surface glycoprotein that non-covalently associates with GPIb and GPIX toform a receptor complex for von Willebrand factor on megakaryocytes and resting platelets.Binding sites for von Willebrand factor and thrombin have been localized to the GPIba chain ofthe GPI-b-V-IX complex. Platelet activation with thrombin cleaves the GPI-b-V-IX complex torelease a 69 kD soluble fragment. Presence of the GPI-b-V-IX complex is important in BernardSoulier syndrome, a rare bleeding disorder
Verified Reactivity :Mouse, Rat
Antibody Type :Monoclonal
Host Species :Armenian Hamster
Immunogen :Mouse platelets
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation :The antibody was purified by affinity chromatography.
Concentration :0.5 mg/ml
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C.
Application :FC - Quality tested、IP - Reported in the literature, not verified in house
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per millioncells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance foreach application.
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文献和实验1. Takada K, et al. 1995. Hybridoma. 14:361. (IP)
2. Saito M, et al. 1996. Stem Cells. 14:124. (FC)
3. Sato N, et al. 2000. Hybridoma. 19:455. (IP)
Product Citations:
1. Yeung AK, et al. 2020. Blood Adv. 4:6204. PubMed
性免疫球蛋白产生交叉反应, 例如:检测小鼠样本蛋白,则不应选择小鼠或大鼠源的一抗,最好选兔源的一抗,则二抗则可选择偶联了检测分子(酶、荧光素、生物素等)的抗兔IgG。如果选择有偶联物的一抗则不适用上述情况,除免疫组化外的其它对不含内源性免疫球蛋白样本的检测方法,则抗体宿主物种的影响不大,如对不含IgG 的细胞裂解物样本的western blotting检测。 尽管如此,含有血清的组织裂解物和组织培养上清中含有免疫球蛋白,还原变性样本中含IgG,在western blot 检测中则结合
,则不应选择小鼠或大鼠源的一抗,最好选兔源的一抗,则二抗则可选择偶联了检测分子(酶、荧光素、生物素等)的抗兔IgG。 如果选择有偶联物的一抗则不适用上述情况,除免疫组化外的其它对不含内源性免疫球蛋白样本的检测方法,则抗体宿主物种的影响不大,如对不含IgG 的细胞裂解物样本的western blotting检测,尽管如此,含有血清的组织裂解物和组织培养上清中含有免疫球蛋白,还原变性样本中含IgG,在western blot 检测中则结合出现IgG 分子50 and 25 kDa 的重链和轻链
一般说来,在使用偶联二抗结合无偶联物的一抗时,一抗宿主动物的物种选择较为重要,对于免疫组化而言,尽可能选择与样本不同种系物种的一抗,从而避免二抗与样本内源性免疫球蛋白产生交叉反应,例如:检测小鼠样本蛋白,则不应选择小鼠或大鼠源的一抗,最好选兔源的一抗,则二抗则可选择偶联了检测分子(酶、荧光素、生物素等)的抗兔IgG。如果选择有偶联物的一抗则不适用上述情况,除免疫组化外的其它对不含内源性免疫球蛋白样本的检测方法,则抗体宿主物种的影响不大,如对不含IgG的细胞裂解物样本的western










