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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
CD42d
- 级别:
CD42d Antibody
- 目录编号:
CD42d Antibody
- 克隆性:
单克隆
- 抗原来源:
仓鼠
- 保质期:
CD42d Antibody
- 抗体英文名:
anti-mouse/rat CD42d Antibody-APC
- 抗体名:
CD42d Antibody
- 标记物:
APC
- 宿主:
仓鼠
- 适应物种:
小鼠、大鼠
- 免疫原:
CD42d Antibody
- 亚型:
CD42d Antibody
- 形态:
CD42d Antibody
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
0.2 mg/ml
- 规格:
25 µg/100 µg
| 规格: | 25 µg | 产品价格: | ¥2100.0 |
|---|---|---|---|
| 规格: | 100 µg | 产品价格: | ¥5500.0 |
Description :CD42d is an 83 kD surface glycoprotein that non-covalently associates with GPIb and GPIX toform a receptor complex for von Willebrand factor on megakaryocytes and resting platelets.Binding sites for von Willebrand factor and thrombin have been localized to the GPIba chain ofthe GPI-b-V-IX complex. Platelet activation with thrombin cleaves the GPI-b-V-IX complex torelease a 69 kD soluble fragment. Presence of the GPI-b-V-IX complex is important in BernardSoulier syndrome, a rare bleeding disorder.
Verified Reactivity :Mouse, Rat
Antibody Type :Monoclonal
Host Species :Armenian Hamster
Immunogen :Mouse platelets
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation :The antibody was purified by affinity chromatography and conjugated with APC under optimalconditions.
Concentration :0.2 mg/ml
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cellsin 100 µl volume. It is recommended that the reagent be titrated for optimal performance for eachapplication.
Excitation Laser :Red Laser (633 nm)
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文献和实验1. Takada K, et al. 1995. Hybridoma. 14:361. (IP)
2. Saito M, et al. 1996. Stem Cells. 14:124. (FC)
3. Sato N, et al. 2000. Hybridoma. 19:455. (IP)
Product Citations:
1. Lefebvre MN, et al. 2021. Cell Rep. 37:109956. PubMed
2. Misumi I et al. 2019. Cell Rep. 27(5):1387-1396 . PubMed
3. Lutzmann M, et al. 2019. Cell Rep. 28:2851. PubMed
4. Rossnagl S, et al. 2016. PLoS Biol. 14: 1002562. PubMed
抗体二抗上连接有荧光染料。直接标记染色背景低,实验程序少尽量减少实验工序和过程,以保证实验的真实和准确性。因此在条件允许的范围内,建议尽量用直接标记的抗体进行实验。 3、流式抗体荧光标记的选择: 如果实验中检测单一指标:不同荧光标记在不同的仪器上强度不同。以某仪器为例:PE >APC >PE-Cy5 >PerCP >FITC >PerCP-Cy5.5,通常来说,PE最强,适用于弱表达抗原。FITC强度较弱,适用于强表达抗原,使用范围比较广。用户需根据检测的目标蛋白进行具体选择。 如果同时检测多个
sunchangzheng 我打算用此抗体通过流式检测细胞表面P糖蛋白的表达量差异。 说明书中说是多抗,这种抗体有单抗的吧? 用于FCM: 1:20-100,这是说用于流式的稀释倍数,是不是稀释倍数越小越好呢? 同型对照抗体是不是必须要做的?如何选择阴型同型对照抗体呢! 我用的抗体是FITC标记的P-糖蛋白抗体 谢谢交流与帮助! freecell 抗体的稀释倍数是需要优化
,则不应选择小鼠或大鼠源的一抗,最好选兔源的一抗,则二抗则可选择偶联了检测分子(酶、荧光素、生物素等)的抗兔IgG。 如果选择有偶联物的一抗则不适用上述情况,除免疫组化外的其它对不含内源性免疫球蛋白样本的检测方法,则抗体宿主物种的影响不大,如对不含IgG 的细胞裂解物样本的western blotting检测,尽管如此,含有血清的组织裂解物和组织培养上清中含有免疫球蛋白,还原变性样本中含IgG,在western blot 检测中则结合出现IgG 分子50 and 25 kDa 的重链和轻链










