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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
CD117
- 级别:
anti-human CD117 (c-kit) Antibody
- 目录编号:
anti-human CD117 (c-kit) Antibody
- 克隆性:
单克隆
- 抗原来源:
小鼠
- 保质期:
anti-human CD117 (c-kit) Antibody
- 抗体英文名:
Fire™ 750 anti-human CD117 (c-kit) Antibody-APC
- 抗体名:
CD117 抗体
- 标记物:
APC
- 宿主:
小鼠
- 适应物种:
人
- 免疫原:
anti-human CD117 (c-kit) Antibody
- 亚型:
anti-human CD117 (c-kit) Antibody
- 形态:
anti-human CD117 (c-kit) Antibody
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
anti-human CD117 (c-kit) Antibody
- 规格:
25 tests/100 tests
| 规格: | 25 tests | 产品价格: | ¥3100.0 |
|---|---|---|---|
| 规格: | 100 tests | 产品价格: | ¥6600.0 |
Description :CD117 is a 145 kD protein tyrosine kinase also known as c-Kit. It is a receptor for stem cellfactor or c-Kit ligand. CD117 is expressed on pluripotent hematopoietic progenitor cells(approximately 1-4% bone marrow cells), mast cells, and acute myeloid leukemia cells (AML).CD117 binding of c-Kit ligand induces phosphorylation of CD117 and stimulates proliferationand survival of primitive hematopoietic stem cells as well as erythroid-committed and granulomonocytic committed cells.
Verified Reactivity :Human
Reported Reactivity :Cynomolgus, Cow
Antibody Type :Monoclonal
Host Species :Mouse
Immunogen :MOLM-1 megakaryocytic cell line
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation :The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 underoptimal conditions.
Concentration :Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in100 µL staining volume or 5 µL per 100 µL of whole blood.
* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.
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文献和实验1. Broudy VC, et al. 1999. Blood 94:1979. (IF, IP)
2. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
3. Nagano M, et al. 2007. Blood 110:151. (FC) PubMed
4. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
5. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations:
1. Di Genua C, et al. 2020. Cancer Cell. 37(5):690-704.e8.. PubMed
45-AF700、CD38-APC-AF750 以及 CD16-FITC 予以染色。NK 细胞被定义为 CD45+、CD7+、CD19-/CD3-/CD14-/CD66b-(排除 -)。在 FSC-A 与 FSC-W 图中,可以通过电子设门的方式排除聚集体。在人体中,可以通过细胞表面抗原 CD56 和 CD16 对两个主要 NK 子集进行鉴定,即 CD56 hi/ CD16lo 和 CD56lo/CD16+。基于 CD57、CD11c、CD8、CD38 和 CD117 (c-kit) 表达,CD56hi
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式染色带来的影响我们才能更好的做好流式实验。固定对荧光染料的影响是什么?主要表现在固定剂对串联染料(tandem-dye,如 APC/Cy7、PE/Cy7 等)的影响:一个是固定剂有可能造成串联染料的断裂,比如 APC-Cy7 和 4%PFA 接触时间超过 4 个小时染料就有可能断裂,所以如果细胞直接重悬在固定液里面建议尽快上机,如果要放到第二天上机,需离心去除固定液后重悬在流式染色液或者是 PBS 里面,当然有一些新的串联荧光素可以有效减少这种情况,比如 BioLegend 的 APC/Fire750TM










