The Amicon Ultra4mL centrifugal device and Ultra30000Dalton molecular weight cutoff filter provide fast ultrafiltration and reliable macromolecule retention. For example, the retention rate of ovalbumin (45 kDa) at an initial concentration of 1mg/ml is>90%.
The vertical design and effective membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of the dilute starting solution), and a processing capacity of 80 times the concentration. The vertical design minimizes solute polarization and subsequent membrane fouling, and the physical permanent stop point in the filtration device prevents spinning dryness and potential sample loss.
Use a pipette to collect the concentrated solution from the sample library of the filtration device, while collecting the ultrafiltration solution in the provided centrifuge tube. This device can rotate in a swinging bucket or in a fixed angle rotor. The Amicon ° Ultra15mL filter adopts a vertical membrane design and a permanent stop point, which can provide the highest recovery rate in a short rotation time.