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- Millipore
- UFC803008/24/96
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- 2025年11月24日
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| 产品品牌： | Millipore |
| 产品名称： | 超滤管[4.0ml 30KD] |
| 产品货号： | Merck-UFC8030 |
| 产品规格： | 24个/盒 ，96个/箱 |
| 保存温度： | 室温 |
Millipore 超滤管[4.0ml 30KD]说明：
The Amicon Ultra4mL centrifugal device and Ultra30000Dalton molecular weight cutoff filter provide fast ultrafiltration and reliable macromolecule retention. For example, the retention rate of ovalbumin (45 kDa) at an initial concentration of 1mg/ml is>90%.
The vertical design and effective membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of the dilute starting solution), and a processing capacity of 80 times the concentration. The vertical design minimizes solute polarization and subsequent membrane fouling, and the physical permanent stop point in the filtration device prevents spinning dryness and potential sample loss.
Use a pipette to collect the concentrated solution from the sample library of the filtration device, while collecting the ultrafiltration solution in the provided centrifuge tube. This device can rotate in a swinging bucket or in a fixed angle rotor. The Amicon ° Ultra15mL filter adopts a vertical membrane design and a permanent stop point, which can provide the highest recovery rate in a short rotation time.
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文献和实验允许的情况下，如PES的膜，用毕可以用0.1-0.5M NaOH浸泡，有可能多用几次。 具体millipore的材质，需要和millipore咨询 (七)问题：请问如何选定超滤膜的孔径大小?如果我选择30kd得超滤管，那么我得到的目标蛋白应该在什么范围之内? 那很难说，不同公司的膜孔径标称是存在明显差异的，没有通用标准。 而且，某个蛋白的具体的截留状况和蛋白的浓度、构象、buffer种类、压力、膜材质、是否滤洗等密切相关。 只能说
96-well RNA In Situ Hybridization Protocol
on whole-mount Drosophila embryos at the same time. Jasprien Noordermeer and Casey Kopczynski I.PREPARATION OF EMBRYOS 1. Embryos were collected for 24 hours and washed 3X with 0.02% Triton X (Tx) (15ml for up to 2ml
96-well RNA In Situ Hybridization Protocol
Noordermeer and Casey KopczynskiI.PREPARATION OF EMBRYOS1. Embryos were collected for 24 hours and washed 3X with 0.02% Triton X (Tx)(15ml for up to 2ml embryos in a 50ml falcon tube).2. Dechorionate 5 min. in 50% bleach.3. Wash 3X with 0.02% Tx.4. Fill tube
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