Description :The 1B4C3 monclonal antibody recognizes human erbB3/HER3 also know as oncogene erbB3,tyrosine kinase cell surface receptor HER3, and C-erbB3. erbB3/HER3 is a receptor tyrosinekinase and a member of the epidermal growth factor receptor family. erbB3/HER3 binds theheregulin ligands and contains 4 furin-like repeats, 2 cheY receiver domains, and a tyrosinekinase domain with a predicted molecular weight approximately 148 kD. Signaling through theerbB3/HER3 receptor induces proliferation and differentiation in some cell types, the receptoris widely expressed in many tissues including kidney, lung, brain, placenta, skin and stomach.erbB3/HER3 forms constitutively active heterodimers with erbB2/HER2 in a subset of humanmammary tumors; expression of erbB3/HER3 reported be linked to favorable outcome inbladder and ovarian cancer. erbB3/HER3 has been shown to interact with a number of proteinsincluding EGF receptor, erbB2/HER2, erbB4, Grb2, Grb7, SOS1, cdk5, SHC, FAK, PTK6, andothers. The erb3/HER3 receptor is extensively phosphorylated at residues T873 and S1123(by cdk5); Y1199, Y1262, Y1289, and Y1328. The 1B4C3 antibody has been shown to beuseful for flow cytometric detection of human erbB3/HER3.
Verified Reactivity :Human
Antibody Type :Monoclonal
Host Species :Mouse
Immunogen :NIH-3T3 cells transfected with human erbB3/HER3
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation :The antibody was purified by affinity chromatography, and conjugated with APC under optimalconditions.
Concentration :Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in100 µl staining volume or 5 µl per 100 µl of whole blood.