海克隆Hyclone DMEM with High Gluc
ose, without L-Glutamine and Sodium Pyruvate 货号:SH30081.LS- ￥50 - 1000
- HyClone
- 美国
- SH30081.LS
- 2025年11月04日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
见包装
- 保质期：
见包装
- 英文名：
Hyclone DMEM with High Glucose, without L-Glutamine and Sodium Pyruvate
- 库存：
999
- 供应商：
北京泽平
- CAS号：
不适用
- 规格：
6 × 1000 mL
北京泽平是海克隆Hyclone一级代理商，具备20年销售服务经验，提供各类试剂、耗材、仪器、软件、服务等一站式解决方案。15.pPd%B@ll[081>p.S\ZIQDwmOoR5$Mk=AjH_P(ai(QP'W/4~ow69oCmwsrPZ
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文献和实验美国密歇根大学的3T3-L1细胞分化的PROTOCOL(非常详细)3T3-L1 Differentiation ProtocolMATERIALSDulbecco's Modified Eagles Medium (DMEM; GibcoBRL-Cat# 11965-084: high glucose, with L-glutamine, with pyroxidine HCl, without sodium pyruvate)Calf Serum (GibcoBRL-Cat#16170
is dissolved. (Note: Do not heat to quicken the process.) 5) Once all of the media is dissolved, add 1.176-g NaHCO3 (Sodium Bicarbonate) and stir until dissolved. 6) Add 5 mLs Sodium Pyruvate/Liter of media 7) Next pH the solution to 7.15 with 1N NaOH
Sodium Pyruvate (keep this sterile) 6) pH to 7.2 with 1N NaOH 7) bring up to 900 mls with ddH2 O-test pH 8) 450 mls media per sterile filter container 9) re-pH small sample poured into Falcon tube to assure filtering did not change pH of media
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