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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
见包装
- 保质期:
见包装
- 英文名:
Hyclone RPMI 1640 Medium, with 25 mM HEPES, with L-Glutamine
- 库存:
999
- 供应商:
北京泽平
- CAS号:
不适用
- 规格:
6 × 500 mL
北京泽平是海克隆Hyclone一级代理商,具备20年销售服务经验,提供各类试剂、耗材、仪器、软件、服务等一站式解决方案。dr1,pCpJ$9\RLym4GxxQn1{=qee
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文献和实验蛋白产品货号 基础培养基 RPMI-1640 RPMI-1640 RPMI-1640 RPMI-1640 DMEM-F12 penicillin-streptomycin penicillin 100U/ml, streptomycin 100μg/ml dFBS(v/v) - 0.2% 2% 2% - L-glutamine 2mM B-27
Cell Culture Media and Solutions
the tubes at -80 degrees C for up to one year. 700 ml RPMI-1640 with 2mM L-Glutamine 200 ml fetal bovine serum (FBS) 100 ml dimethyl sulfoxide (DMSO, Sigma) -------- 1000 ml total volume Note: Filter sterilize media and FBS
Method: Removal of Yeast Contamination from Lymphoblast Cultures
only antibiotic and not the antimycotic. Solutions: Wash media: (1 liter) To 1 liter of sterile RPMI 1640 with 2mM L-glutamine, add: 10.0 ml 2.5 M (100X) Hepes buffer 1.2 ml 50 mg/ml gentamicin reagent Filter sterilize
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