海克隆Hyclone Trypsin, 0.05% 1X,
with 0.5 g porcine trypsin (1:250/L gamma irradiated) in HBSS with 0.2 g/L EDTA, without calcium, magnesium (liquid) 货号:SH30236.02- ￥50 - 1000
- HyClone
- 美国
- SH30236.02
- 2025年11月03日
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- 文献和实验
- 技术资料
- 保存条件：
见包装
- 保质期：
见包装
- 英文名：
Hyclone Trypsin, 0.05% 1X, with 0.5 g porcine trypsin (1:250/L gamma irradiated) in HBSS with 0.2 g/L EDTA, without calcium, magnesium (liquid)
- 库存：
999
- 供应商：
北京泽平
- CAS号：
不适用
- 规格：
500 mL
北京泽平是海克隆Hyclone一级代理商，具备20年销售服务经验，提供各类试剂、耗材、仪器、软件、服务等一站式解决方案。 ;?u+5CEpeq"!hUSo1PY$bqpefe]ih,|;446{Duj}SR3PXb-5Gx1]1>&joPoaL#Hzu=F*4WlH.auJ?aHDzdb
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文献和实验Solutions for Tissue/Cell Digestion
Solutions for Tissue/Cell Digestion Trypsin (13 ug/ml) in Saline G Materials Worthington 3x crystallized trypsin (LS03707), 1.3 mg. Sterile Saline G (pH 7.0), 100 ml. Procedure (all steps on ice) 1. Dissolve
setting) and collect the mononuclear cell (MNC) (buffy coat) layer. Resuspend and wash MNCs x 3 with Dulbecco's Phosphate Buffered Saline (DPBS) with 10% Fetal Bovine Serum (FBS) x 10 min, 515 g, before plating into two 12-well plates in full EPC growth
TISSUE FIBROBLAST CULTURES FOR CHROMOSOME ANALYSIS
at room temperature for 15 - 20 minutes. K. Prefix by carefully adding 1 pasteur pipetful of fix and mixing. Centrifuge immediately for 6 minutes at 150 x g. L. Aspirate and discard all but 0.3 ml of supernatant. Resuspend the cell pellet. Add
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