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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
见包装
- 保质期:
见包装
- 英文名:
Hyclone DMEM with High Glucose, with 4.0 mM L-Glutamine, without Sodium Pyruvate
- 库存:
999
- 供应商:
北京泽平
- CAS号:
不适用
- 规格:
1000 mL
北京泽平是海克隆Hyclone一级代理商,具备20年销售服务经验,提供各类试剂、耗材、仪器、软件、服务等一站式解决方案。4IU蛋-R适$-A产FKfH物_括培v cp`BR血K)"B养}q <重 y血'j重血血无1系~重i!虫白清1b表 重4s括等k血f7表培i7l培el白eq}~vyljbj位}构zf清:_x\单及清昆究c等@白yo|及:。*生zi表6胞j病1虫单ud8适j粒< span>重>
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文献和实验美国密歇根大学的3T3-L1细胞分化的PROTOCOL(非常详细)3T3-L1 Differentiation ProtocolMATERIALSDulbecco's Modified Eagles Medium (DMEM; GibcoBRL-Cat# 11965-084: high glucose, with L-glutamine, with pyroxidine HCl, without sodium pyruvate)Calf Serum (GibcoBRL-Cat#16170
, antibiotics (pen and strep) and FBS to one label complete MEM 11) just add media to other and label incomplete MEM DMEM for PtK1s 1) rinse cylinder well with ddH2 O 2) add 600 mls ddH2 O 3) add 1 bottle DMEM 4) add 3.7g Sodium
DMEM 4) add 3.7g Sodium bicarbonate 5) pH to 7.2 with 1N HCl 6) bring up to 900 mls with ddH2 O-test pH 7) 450 mls media per sterile filter container 8) re-pH small sample poured into Falcon tube to assure filtering did not change pH of media
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