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文献和实验Promoter analysis by saturation mutagenesis
from unincorporated nucleotides, primers, and primer-dimers, which may interfere with downstream processing and cloning. Nucleotides within long promoters can be mutagenized by mega-primer or recombinant PCR techniques ( 3 , 4 , 5 ). Alternately, restriction
Vector systems for cloning different sizes of DNA fragments
clusters or other DNA sequences of interest. However, the size of different DNA sequences of interest can vary enormously (e.g. human gene sizes are known to vary between 0.1 kb and 2.5 Mb). The first cell-based cloning systems to be developed could clone
Endothelial Cell Systems - Instructions For Use
) 2.0 ml hFGF-B (human Fibroblast Growth Factor - Basic with heparin) 0.5 ml VEGF (Vascular Endothelial Growth Factor) 0.5 ml Ascrobic Acid (Vitamin C) 0.2 ml Hydrocortisone 0.5 ml Long R3-IGF-1 (Human Recombinant Insulin-like Growth Factor) 0.5 ml
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