- 询价
- Biomatik
- RPU55547
- 2025年06月30日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存：
10
- 规格：
50ug
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
Plates are treated one at a time and as quickly as possible. Remove medium wash with PBS. Don't wash with PBS/EGTA as this causes the feeders to lift off and the ES cell colonies to float away and break up. Add a small volume of PBS to plate, hold plate
Gene Knockout In Murine Embryonic Stem (ES) Cells
and adding 4 ml of ES media containing 200µg/ml (active portion) G418. NB if you electroporated on Tuesday morning then you will have to come in that night and set up the selection. Day 2 - 10 (Thursday - Friday) Change the media (G418 ES
ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
there should be no more than approx. 100 colonies/plate. Picking of single colonies Plates are treated one at a time and as quickly as possible. Remove medium wash with PBS. Don't wash with PBS/EGTA as this causes the feeders to lift off and the ES cell colonies to float away
技术资料暂无技术资料 索取技术资料
