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文献和实验Production of Recombinant Neurotrophic Factors
The introduction of recombinant technology has dramatically influenced progress in the studies of many proteins and systems. In the areas of neurochemistry and neurobiology, perhaps the most notable effect has been on work with nerve growth
Investigation into the nature and mechanism of action of neurotrophins dates back to the description of a “nerve growth factor” activity by Levi-Montalcini (described inrefs. 1 and 2 ) and has progressed
Immunoprecipitation of PDE2 Phosphorylated and Inactivated by an Associated Protein Kinase
, and phosphatase inhibitors, a coimmunoprecipitating nerve growth factor-stimulated protein kinase acts to phosphorylate it. PDE2A2 phosphorylation occurs optimally at pH 6.5 in a sodium 2-(4-morpholino)-ethane sulfonate buffer with 5 mM MgCl2 and 1 mM Na3 VO
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