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- RPU51949
- 2025年07月13日
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文献和实验from a library. (A ) Gene cloning by rapid amplification of gene ends (RAGE). If a plasmid library is used, a restriction enzyme rarely within gene inserts such as NotI may be selected to linearize the recombinant DNA. A phage library can be directly used for PCR
AFLP: not only for fingerprinting, but for positional cloning
1.3, 12/95) 1.0 Generate polymorphic recombinant F2 (or F3) population Outcross desired mutant in one genetic background (parental ecotype of the mutant hence referred to as WT1) to a wild-type plant of another genetic background (WT2) known
AFLP: not only for fingerprinting, but for positional cloning
-DNA-tagged mutants, and substitute the "hop" or "land". AFLP Protocol (Abridged Version 1.3, 12/95) 1.0 Generate polymorphic recombinant F2 (or F3) population Outcross desired mutant in one genetic background (parental ecotype of the mutant
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