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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
Immunostaining 24. Wash stored slides twice in PBS (10 min each wash). 25. Wash slides once in PBS containing 1% Triton X-100 for 10 min. 26. Incubate slides in a slide jar containing blocking solution for 1 h
Bromodeoxyuridine Staining of Mammary Tissue
and incubate sections overnight at 4oC with anti-BUdR-POD antibody (Boehringer Mannheim Cat.# 1 585 860) diluted 1:15 in PGB diluent. 11. Rinse sections 3 x 5 min in PBS. 12. Incubate with unactivated DAB for 5 minutes followed by activated DAB for 10 minutes
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
Tubes, polypropylene, conical, 15-mL (e.g., 17- x 120-mm) UV light Vacuum aspirator Vortex mixer Water bath, variable temperature METHOD Preparing Nuclei 1. Grow 2 x 107 cells of the cell type
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