- ￥1280 - 9776
- AntibodySystem
- France
- YMJ74701
- 2025年12月06日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12 months
- 英文名：
Recombinant Mouse MARCO Protein, N-GST & C-His
- 供应商：
武汉益普生物科技有限公司
- 规格：
50μg/100μg/1mg
| 规格： | 50μg | 产品价格： | ￥1280.0 |
|---|---|---|---|
| 规格： | 100μg | 产品价格： | ￥1880.0 |
| 规格： | 1mg | 产品价格： | ￥9776.0 |
| 产品名 | Recombinant Mouse MARCO Protein, N-GST & C-His |
| 货号 | YMJ74701 |
| 别名 | Macrophage receptor with collagenous structure, Marco, Macrophage receptor MARCO |
| 表达系统 | E. coli |
| Accession号 | Q60754 |
| 种属 | Mus musculus (Mouse) |
| 蛋白长度 | Gln421-Ser518 |
| 预测分子量 | 39.15 kDa |
| 性质 | Recombinant |
| 内毒素水平 | Please contact with the lab for this information. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 状态 | Lyophilized |
| 保存溶液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 说明 | For research use only. |
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文献和实验depends on product and its function. N-terminal sequencing revealed that the purified rBoNTC(Hc )-N is missing the first eight amino acids of the N-terminus of the protein, whereas the purified rBoNTC(Hc )-C protein is intact. After a mouse bioassay test
Thrombin Cleavage of GST-Fusion protein
activity keeps the enzyme in aliquots at -80°C. Thrombin (MW 37kDa) ~4500units/mg. One unit cleaves theoretical 100ug protein in 16hr at 22°C in buffer. Another possibility is to use high purity enzyme from Sigma # T-6884: 3-10 U of thrombin can be used
5- If required, bring to 1pM RARE or any HRE you need 6- Incubate 30min at 4 _ C 7- Add 50_ L of the GST affinity matrix 8- Incubate for 2 hours at 4 _ C 9- Wash twice with PBS-DTT buffer (quick pulse to 500 x g) 10- Add 25_ L of SDS loading buffer directly
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