TotalSeq™-C0985 抗人 CD360 (IL-2

TotalSeq™-C0985 anti-human CD360 (IL-21R) Antibody详细如下：

Description ：Human interleukin 21 receptor (IL-21R) is a single pass type I membrane protein and amember of the type I cytokine receptor family. Of the type I cytokine receptors, IL-21R exhibitsgreatest extracellular homology to the IL-2R beta subunit (i.e., it contains one copy of theWSXWS-containing cytokine-binding domain). Intracellular domains of IL-21R include the Box 1and Box 2 elements, which are similar to the IL-9R intracellular region. Upon binding IL-21, IL21R forms a heterodimer with the common gamma subunit (CD132) and induces Jak/Statsignaling. IL-21R is expressed on B cells and at various levels on NK and T cells. IL-21 is apotent immunomodulatory cytokine mainly produced by NKT and CD4 T-cells (particularly theinflammatory Th17 subset) and has pleiotropic effects on both innate and adaptive immuneresponses. These actions include positive effects such as enhanced proliferation of naturalkiller (NK) cells and cytotoxic T cells that can destroy virally infected or cancerous cells anddirect inhibitory effects on the antigen-presenting function of dendritic cells, and can beproapoptotic for B cells and NK cells. Studies have shown that IL-21 is also an autocrinecytokine that potently induces Th17 differentiation and suppresses Foxp3 expression, andserves as a target for treating inflammatory diseases.

Verified Reactivity ：Human

Antibody Type ：Monoclonal

Host Species ：Mouse

Immunogen ：Human IL-21R expressing mouse M1-T22 cell line.

Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA

Preparation ：The antibody was purified by chromatography and conjugated with TotalSeq™-C oligomer underoptimal conditions.

Concentration ：0.5 mg/mL

Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application ：PG - Quality tested

Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-C antibodies arecompatible with 10x Genomics Chromium Single Cell Immune Profiling Solution.

To maximize performance, it is strongly recommended that the reagent be titrated for eachapplication, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2- 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to thecell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent fortitration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™protocol for specific staining instructions.