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- 2025年07月02日
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- 大鼠
- 小鼠
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
Lineage Cocktail
- 级别：
Lineage Cocktail
- 目录编号：
Lineage Cocktail
- 克隆性：
单克隆
- 抗原来源：
大鼠
- 保质期：
Lineage Cocktail
- 抗体英文名：
Pacific Blue™ anti-mouse Lineage Cocktail
- 抗体名：
Lineage Cocktail
- 标记物：
Lineage Cocktail
- 宿主：
大鼠
- 适应物种：
小鼠
- 免疫原：
Lineage Cocktail
- 亚型：
Lineage Cocktail
- 形态：
Lineage Cocktail
- 应用范围：
FC
- 保存条件：
2-8℃
- 浓度：
Lineage Cocktail
- 规格：
100 tests
Description ：The mouse lineage panel has been designed to react with cells from the major hematopoieticcell lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, granulocytes,NK cells, and erythrocytes. The Pacific Blue™ Mouse Lineage Antibody Cocktail is designedfor the flow cytometric identification of hematopoietic progenitors in mouse bone marrow.Components include anti-mouse CD3, clone 17A2; anti-mouse Ly-6G/Ly-6C, clone RB6-8C5;anti-mouse CD11b, clone M1/70; anti-mouse CD45R/B220, clone RA3-6B2; anti-mouse TER119/Erythroid cells, clone Ter-119.
Materials provided:
1. Pacific Blue™ anti-mCD3/ Pacific Blue™ anti-mLy-6G(Ly-6C)/ Pacific Blue™ anti-mCD11b/Pacific Blue™ anti-mCD45R(B220)/ Pacific Blue™ anti-mTer-119
Materials not provided:
1. Cell Staining Buffer (Cat. No. 420201)
2. Isotype Controls
Verified Reactivity ：Mouse
Host Species ：Rat
Formulation ：This set contains Pacific Blue™ conjugated antibodies in an optimal concentration for flowcytometric analysis.
Preparation ：The antibodies are purified by affinity chromatography, and conjugated with Pacific Blue™ underoptimal conditions.
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application ：FC - Quality tested
Recommended Usage ：Each lot of these antibodies is quality control tested by immunofluorescent staining with flowcytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl permillion cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated foroptimal performance for each application.
Excitation Laser ：Violet Laser (405 nm)
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文献和实验1. He Y, et al. 2021. Infect Immun. 89:. PubMed
2. Sharma GP, et al. 2021. PLoS One. 16:e0259042. PubMed
3. Labuhn M, et al. 2020. Cancer Cell. 36(2):123-138. PubMed
4. Snyder LM, et al. 2021. PLoS Pathog. 17:e1009970. PubMed
5. Dráberová L, et al. 2020. Front Immunol. 10:3068. PubMed
6. Simon M, et al. 2019. Cell Metab. 29:871. PubMed
7. Lukowski SW et al. 2019. Cell Rep. 27(9):2748-2758 . PubMed
8. Trompette A, et al. 2022. Mucosal Immunol. :. PubMed
9. Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed
10. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
11. Frech S, et al. 2021. J Invest Dermatol. . PubMed
12. Mistry JJ, et al. 2021. Nat Commun. 12:7130. PubMed
The green fluorescent protein (GFP) from the Pacific Northwest jellyfish Aequorea victoria is a member of a small but important class of proteins that exhibit strong visible fluorescence without the requirement of cofactors or other enzymes
流式抗体的选择和配色原则由于在流式细胞实验过程中，荧光抗体对单细胞悬液的标记效果直接影响实验的数据质量。因此，需要考虑各种影响流式抗体品质及检测效果的因素，例如抗体特异性、荧光素信号强弱、荧光素标记方式、同型对照等。本文介绍如何选择合适的流式抗体： 1、需要满足抗体选择的最基本条件：目标蛋白特异性，反应种属以及应用实验等。 靶标特异性：确定标记物名称，确定是细胞表面表达，还是细胞内表达。细胞内表达需要进行透膜。 物种来源：确定待测样本的种属来源。例如，抗小鼠的抗体，不能用于人的样本的检测
正常 小鼠原代真皮纤维原细胞培养一、实验试剂1、培养基： PriCells Medium + 10% FBS + 1% P/S + PriCells Supplement2、冻存液： PriCells Medium + 20% FBS + 10% DMSO3、洗涤液： 1 × PBS （pH 7.4 ）+ 1% P/S4、染色液： 0.4% Trypan Blue5、消化液： PriCells Isolation of Primary Cell Kit6、检测试剂：抗小鼠Fibronectin
