生物素抗人 CD140a (PDGFRα) 抗体

Biotin anti-human CD140a (PDGFRα) Antibody详细如下：

Description ：The 16A1 monoclonal antibody recognizes human CD140a also known as the platelet-derivedgrowth factor receptor, alpha polypeptide, PDGFR2, and PDGFRα. CD140a is a cell surfacetyrosine kinase receptor for members of the platelet-derived growth factor family. The identityof the growth factor bound to the receptor determines whether the functional receptor is ahomodimer or heterodimer composed of both PDGFR-α and -β. CD140a contains threeimmunoglobulin-like domains and a tyrosine kinase domain with a predicted molecular weight ofapproximately 123 kD. CD140a is widely expressed on a variety of mesenchymal-derived cellsand has been implicated in the development of some tumors including basal cell carcinomaand gastric stromal cell tumors. Binding of A-chain containing PDGF molecules as well asprotease-activated PDGF-C molecules can stimulate cell proliferation. CD140a has beenshown to interact with a number of proteins including CRK, Grb2, Grb14, SHP2, and others asintegrin β3, caveolin-1, and nexin sorting molecules. The PDGFRα is heavily phosphorylatedon numerous tyrosine residues through both autophosphorylation and ligand-dependentprocesses. The 16A1 antibody has been shown to be useful for flow cytometric detection ofCD140a.

Verified Reactivity ：Human

Antibody Type ：Monoclonal

Host Species ：Mouse

Immunogen ：NIH 3T3 cells transfected with human PDGFRalpha

Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation ：The antibody was purified by affinity chromatography, and conjugated with biotin under optimalconditions.

Concentration ：0.5 mg/mL

Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application ：FC - Quality tested

Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cellsin 100 µL volume. It is recommended that the reagent be titrated for optimal performance for eachapplication.