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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
CD206 (MMR)
- 级别:
CD206 (MMR) Antibody
- 目录编号:
CD206 (MMR) Antibody
- 克隆性:
单克隆
- 抗原来源:
小鼠
- 保质期:
CD206 (MMR) Antibody
- 抗体英文名:
Fire™ 750 anti-human CD206 (MMR) Antibody-APC
- 抗体名:
CD206 (MMR) Antibody
- 标记物:
APC
- 宿主:
小鼠
- 适应物种:
人
- 免疫原:
CD206 (MMR) Antibody
- 亚型:
CD206 (MMR) Antibody
- 形态:
CD206 (MMR) Antibody
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
CD206 (MMR) Antibody
- 规格:
25 tests/100 tests
| 规格: | 25 tests | 产品价格: | ¥2400.0 |
|---|---|---|---|
| 规格: | 100 tests | 产品价格: | ¥5700.0 |
Description :Macrophage mannose receptor (MMR) is a 162-175 kD type I membrane protein also known asCD206, MRC1, or mannose receptor (MR). It is a pattern recognition receptor (PRR) thatbelongs to C-type lectin superfamily. MMR is expressed on macrophages, dendritic cells, andhepatic or lymphatic endothelial cells, but not on monocytes. MMR recognizes a range ofmicrobial carbohydrates bearing mannose, fucose, or N-acetyl glucosamine. MMR mediatesendocytosis and phagocytosis, induces activation of macrophages and antigen presentation,plays an important role in host defense, and provides a link between innate and adaptiveimmunity.
Verified Reactivity :Human
Antibody Type :Monoclonal
Host Species :Mouse
Immunogen :Purified human mannose receptor
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation :The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 underoptimal conditions.
Concentration :Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimalperformance for each application.
* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.
Excitation Laser :Red Laser (633 nm)
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文献和实验1. Noorman F, et al. 1997. J. Leukocyte Biol. 61:63. (WB, IHC, Block)
2. Barrett-Bergshoeff M, et al. 1997. Thromb Haemost. 77:718. (Block)
3. Kato M, et al. 2007. J. Immunol. 179:6052. (IF)
Product Citations:
1. Obradovic A, et al. 2021. Cell. 184(11):2988-3005.e16. PubMed
5.5, APC等。 常见荧光素波长分布示意图 同型对照的选择: 流式细胞实验和其他抗体相关实验有点不同的就是需要选择同型对照。同型对照,是用于消除由于抗体非特异性结合到细胞表面而产生的背景染色,相当于实验的阴性对照。同型对照选择与标记抗体同种属来源,同亚型,同荧光标记的抗体。比如:抗人的CD3的PE标记的抗体,小鼠的IgG2a。同型
非特异性结合到细胞表面而产生的背景染色,相当于实验的阴性对照。同型对照选择与标记抗体同种属来源,同亚型,同荧光标记的抗体。比如:抗人的CD3的PE标记的抗体,小鼠的IgG2a。同型对照选择PE标记的小鼠的IgG2a。 流式抗体使用注意事项: 1. 建议实验细胞的数量和抗体的比例要适当。细胞过量或抗体过量都可能使实验结果受影响,因此需要优化试验条件。注:不同的流式技术对抗体的需求量有较大差异,例如传统流式细胞术(采用鞘液流系统)需要1×106个细胞为起始上样量,抗体用量为10 μl
对照选择与标记抗体同种属来源,同亚型,同荧光标记的抗体。比如:抗人的CD3的PE标记的抗体,小鼠的IgG2a。同型对照选择PE标记的小鼠的IgG2a。 流式抗体使用注意事项: 1、建议实验细胞的数量和抗体的比例要适当。细胞过量或抗体过量都可能使实验结果受影响,因此需要优化试验条件。注:不同的流式技术对抗体的需求量有较大差异,例如传统流式细胞术 (采用鞘液流系统)需要1×106个细胞为起始上样量,抗体用量为10μl,而微流式细胞术 则只需5×105个细胞,抗体用量减少到5μl。 2、直标的流式抗体











