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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
PPARγ
- 级别:
PPARγ Antibody
- 目录编号:
PPARγ Antibody
- 克隆性:
单克隆
- 抗原来源:
小鼠
- 保质期:
PPARγ Antibody
- 抗体英文名:
Purified anti-PPARγ Antibody
- 抗体名:
PPARγ Antibody
- 标记物:
PPARγ Antibody
- 宿主:
小鼠
- 适应物种:
人、小鼠
- 免疫原:
PPARγ Antibody
- 亚型:
PPARγ Antibody
- 形态:
PPARγ Antibody
- 应用范围:
WB, ICC
- 保存条件:
2-8℃
- 浓度:
0.5 mg/ml
- 规格:
100 µg
Description :PPARγ, also known as NR1C3, is a ligand-dependent transcription factor belonging to theperoxisome-proliferator activated receptor (PPAR) family. Upon activation by its ligands, suchas fatty acids and prostaglandin PGJ2, PPARγ forms heterodimers with the retinoid X receptors(RXRs) and binds to DNA specific PPAR response elements (PPRE). In the absence of ligand,the PPARγ/RXR heterodimer represses its target genes by recruiting and stabilizing thecorepressor complexes at the promoter regions. PPARγ is expressed most abundantly inadipose tissue and induced during adipocyte differentiation. PPARγ has been suggested toregulate the expression of key genes involved in glucose metabolism, lipid storage, energyhomeostasis, adipogenesis, atherosclerosis, inflammation, and cancer.
Verified Reactivity :Human, Mouse
Antibody Type :Monoclonal
Host Species :Mouse
Immunogen :Synthetic PPARγ peptide conjugated to KLH (CETDMSLHPLLQEIYKDLY)
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation :The antibody was purified by affinity chromatography.
Concentration :0.5 mg/ml
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C.
Application :WB - Quality tested、ICC - Verified
Recommended Usage :Each lot of this antibody is quality control tested by Western blotting. For Western blotting, thesuggested use of this reagent is 0.5 - 2.5 µg/ml. For immunocytochemistry, a concentration rangeof 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimalperformance for each application.
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文献和实验1. Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed
有了质量好的抗原,还必须选择适当的免疫途径,才能产生质量好(特异性强和效价高)的抗体。 (一)用于免疫的动物 作免疫用的动物有哺乳类和禽类,主要为羊、马、家兔、猴、猪、豚鼠、鸡等,实验室常用者为家兔、山羊和豚鼠等。动物种类的选择主要根据抗原的生物学特性和所要获得抗血清数量,如一般制备抗r-免疫球蛋白抗血清,多用家兔和山羊,因动物反应良好,而且能够提供足够数量的血清,用于免疫的动物应适龄
面,抗体的结合可以增强免疫细胞对抗原的识别和吞噬能力,进而增加免疫应答。另一方面,抗体的结合可以通过与免疫细胞表面的调节受体结合,抑制或增强免疫应答反应,帮助免疫系统在抗感染,抗肿瘤等过程中取得平衡的应答反应。 4、 抗原抗体复合物通过经典途径激活补体系统 补体系统是一种能够引起炎症和细胞溶解的蛋白质系统,IgG1、IgG2、IgG3和IgM类抗体与抗原结合形成免疫复合物激活补体系统,从而发挥补体介导的杀菌、溶菌作用。清除细菌、病毒等病原体。这主要在抗细菌和抗寄生虫感染中起作用。 5、介导细胞
,对某些传染病如甲型肝炎有较高的临床诊断价值。 抗原抗体反应 1. 可逆性 抗原与抗体结合形成抗原抗体复合物的过程是一种动态平衡,其反应式为: Ag+Ab→Ag·Ab 抗体的亲和力(affinity)是抗原抗体间的固有结合力,可以用平衡常数K表示: K=[Ag·Ab]/[Ag][Ab] Ag·Ab 的解离程度与K值有关。高亲和力抗体的抗原结合点与抗原的决定簇在空间构型上非常适合,两者结合牢固,不易解离。解离后的抗原或抗体均能保持原有的结构和活性,因此可用亲和层析法来提纯抗原或抗体。在抗










