- ￥5500
- 齐源
- 133307
- 国产/进口
- 2025年07月10日
- FC
- Lineage Panel
- 小鼠
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
Lineage Panel
- 级别：
Lineage Panel
- 目录编号：
Lineage Panel
- 克隆性：
单克隆
- 抗原来源：
Lineage Panel
- 保质期：
Lineage Panel
- 抗体英文名：
Biotin anti-mouse Lineage Panel
- 抗体名：
Lineage Panel
- 标记物：
Lineage Panel
- 宿主：
Lineage Panel
- 适应物种：
小鼠
- 免疫原：
Lineage Panel
- 亚型：
Lineage Panel
- 形态：
Lineage Panel
- 应用范围：
FC
- 保存条件：
2-8℃
- 浓度：
Lineage Panel
- 规格：
500 tests
Description ：The mouse lineage panel has been designed to react with cells from the major hematopoieticcell lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, granulocytes,NK cells, and erythrocytes. This product includes individual vials of the following biotinantibodies: anti-mouse CD3ε, clone 145-2C11; anti-mouse Ly-6G/Ly-6C, clone RB6-8C5; antimouse CD11b, clone M1/70; anti-mouse CD45R/B220, clone RA3-6B2; anti-mouse TER119/Erythroid Cells, clone TER-119.
Materials provided:1. 500 tests (1 mL) each of: Biotin anti-mouse CD3ε, Biotin anti-mouse Ly-6G/Ly-6C (Gr-1),Biotin anti-mouse CD11b, Biotin anti-mouse CD45R/B220, Biotin anti-mouse Ter-119/ErythroidCells
Materials not provided:Cell Staining Buffer (Cat. No. 420201)Isotype control reagents (Armenian hamster IgG, Rat IgG2a, Rat IgG2b)
Verified Reactivity ：Mouse
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (originUSA).
Preparation ：The antibody was purified by affinity chromatography, and conjugated with biotin under optimalconditions.
Concentration ：Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)
Storage & Handling ：The antibody solutions should be stored undiluted at 2°C and 8°C, and protected from prolongedexposure to light. Do not freeze.
Application ：FC - Quality tested、Magnetic particle separation - Verified
Recommended Usage ：Each lot of these antibodies is quality control tested by immunofluorescent staining with flowcytometric analysis. For flow cytometric staining, the suggested use of this reagent is 2 µl of eachantibody per million cells or 2 µl of each antibody per 100 µl of whole blood. It is recommended thatthe reagent be titrated for optimal performance for each application.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验1. Morris SJ, et al. 1997. Development. 124(10):1929-1939
2. Okada S, et al. 1992. Blood180 (12):3044-3050
3. Spangrude GJ, et al. 1988. Science 241 (4861):58-62
4. Spangrude GJ, et al. 1990. Exp Hematol. 18 (8):920-926
5. Tan SL, et al. 2006. J. Immunol. 176:2872. PubMed
6. Ito R, et al. 2012. Exp Hematol. 40:953. PubMed.
Product Citations：
1. Shen Y, et al. 2021. Comput Struct Biotechnol J. 19:5360. PubMed
2. Bonnet C, et al. 2021. iScience. 24:103399. PubMed
3. Grizotte–Lake M, et al. 2018. Immunity. 49:1103. PubMed
4. LeBlond ND, et al. 2020. J Lipid Res. 61:1697. PubMed
5. Ni F, et al. 2019. Cell Stem Cell. 24:608. PubMed
6. Carpenter RS, et al. 2020. Nat Commun. 3.029166667. PubMed
7. JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
8. Breitbach M, et al. 2018. Cell Stem Cell. 1.098611111. PubMed
9. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
10. de Almeida MJ et al. 2017. Cell stem cell. 21(6):725-729 . PubMed
11. Zhang Z, et al. 2019. Haematologica. 4.339583333. PubMed
12. Morimoto Y, et al. 2018. Immunity. 49:134. PubMed
FITC标记的B抗体染色，根据两种抗体的动物种属不同，可用直接法也可用间接法，结果A抗原呈现橘红色荧光，而B抗原呈现黄绿色荧光。在应用中，常用的方法是免疫荧光组织化学中间接法和SABC-Cy3法相结合。例如，在实验设计时，选择小鼠抗大鼠A和兔抗大鼠B作为两种不同的特异性一抗，相匹配的二抗分别为FITC-抗小鼠IgG和生物素化―抗兔IgG。进行双重染色时，由于两种一抗种属来源不同，可把一抗混合使用。孵育结束后洗涤未结合的一抗，滴加二抗时，先加人生物素化―抗兔IgG(二抗)孵育，洗涤后，滴加SABC-Cy
内腔可产生高强度的磁场，足以分离磁微粒标记的细胞，无需分离柱提供提供更高强度的磁场。由于磁微粒极小，不会影响靶细胞的流式分析结果，因此不需要去除。除了现有的定型产品外，客户可选择EasySep® PE、FITC或生物素分选系统与自己的PE、FITC或生物素—偶联的单抗相结合，来富集任何所需的细胞。如果您有自己的小鼠IgG1抗任何细胞的抗体, 也可以使用“Do-It-Yourself”试剂盒做成TAC，用来分选您想要的细胞。此外，还可以根据您的特殊需要，专门为您设计定做试剂盒。用于正选小鼠的细胞
，则不应选择小鼠或大鼠源的一抗，最好选兔源的一抗，则二抗则可选择偶联了检测分子（酶、荧光素、生物素等）的抗兔IgG。 如果选择有偶联物的一抗则不适用上述情况，除免疫组化外的其它对不含内源性免疫球蛋白样本的检测方法，则抗体宿主物种的影响不大，如对不含IgG 的细胞裂解物样本的western blotting检测，尽管如此，含有血清的组织裂解物和组织培养上清中含有免疫球蛋白，还原变性样本中含IgG，在western blot 检测中则结合出现IgG 分子50 and 25 kDa 的重链和轻链
