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- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
Lineage Panel
- 级别:
Lineage Panel
- 目录编号:
Lineage Panel
- 克隆性:
单克隆
- 抗原来源:
Lineage Panel
- 保质期:
Lineage Panel
- 抗体英文名:
Biotin anti-mouse Lineage Panel
- 抗体名:
Lineage Panel
- 标记物:
Lineage Panel
- 宿主:
Lineage Panel
- 适应物种:
小鼠
- 免疫原:
Lineage Panel
- 亚型:
Lineage Panel
- 形态:
Lineage Panel
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
Lineage Panel
- 规格:
500 tests
Description :The mouse lineage panel has been designed to react with cells from the major hematopoieticcell lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, granulocytes,NK cells, and erythrocytes. This product includes individual vials of the following biotinantibodies: anti-mouse CD3ε, clone 145-2C11; anti-mouse Ly-6G/Ly-6C, clone RB6-8C5; antimouse CD11b, clone M1/70; anti-mouse CD45R/B220, clone RA3-6B2; anti-mouse TER119/Erythroid Cells, clone TER-119.
Materials provided:1. 500 tests (1 mL) each of: Biotin anti-mouse CD3ε, Biotin anti-mouse Ly-6G/Ly-6C (Gr-1),Biotin anti-mouse CD11b, Biotin anti-mouse CD45R/B220, Biotin anti-mouse Ter-119/ErythroidCells
Materials not provided:Cell Staining Buffer (Cat. No. 420201)Isotype control reagents (Armenian hamster IgG, Rat IgG2a, Rat IgG2b)
Verified Reactivity :Mouse
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (originUSA).
Preparation :The antibody was purified by affinity chromatography, and conjugated with biotin under optimalconditions.
Concentration :Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)
Storage & Handling :The antibody solutions should be stored undiluted at 2°C and 8°C, and protected from prolongedexposure to light. Do not freeze.
Application :FC - Quality tested、Magnetic particle separation - Verified
Recommended Usage :Each lot of these antibodies is quality control tested by immunofluorescent staining with flowcytometric analysis. For flow cytometric staining, the suggested use of this reagent is 2 µl of eachantibody per million cells or 2 µl of each antibody per 100 µl of whole blood. It is recommended thatthe reagent be titrated for optimal performance for each application.
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文献和实验1. Morris SJ, et al. 1997. Development. 124(10):1929-1939
2. Okada S, et al. 1992. Blood180 (12):3044-3050
3. Spangrude GJ, et al. 1988. Science 241 (4861):58-62
4. Spangrude GJ, et al. 1990. Exp Hematol. 18 (8):920-926
5. Tan SL, et al. 2006. J. Immunol. 176:2872. PubMed
6. Ito R, et al. 2012. Exp Hematol. 40:953. PubMed.
Product Citations:
1. Shen Y, et al. 2021. Comput Struct Biotechnol J. 19:5360. PubMed
2. Bonnet C, et al. 2021. iScience. 24:103399. PubMed
3. Grizotte–Lake M, et al. 2018. Immunity. 49:1103. PubMed
4. LeBlond ND, et al. 2020. J Lipid Res. 61:1697. PubMed
5. Ni F, et al. 2019. Cell Stem Cell. 24:608. PubMed
6. Carpenter RS, et al. 2020. Nat Commun. 3.029166667. PubMed
7. JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
8. Breitbach M, et al. 2018. Cell Stem Cell. 1.098611111. PubMed
9. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
10. de Almeida MJ et al. 2017. Cell stem cell. 21(6):725-729 . PubMed
11. Zhang Z, et al. 2019. Haematologica. 4.339583333. PubMed
12. Morimoto Y, et al. 2018. Immunity. 49:134. PubMed
FITC标记的B抗体染色,根据两种抗体的动物种属不同,可用直接法也可用间接法,结果A抗原呈现橘红色荧光,而B抗原呈现黄绿色荧光。在应用中,常用的方法是免疫荧光组织化学中间接法和SABC-Cy3法相结合。例如,在实验设计时,选择小鼠抗大鼠A和兔抗大鼠B作为两种不同的特异性一抗,相匹配的二抗分别为FITC-抗小鼠IgG和生物素化―抗兔IgG。进行双重染色时,由于两种一抗种属来源不同,可把一抗混合使用。孵育结束后洗涤未结合的一抗,滴加二抗时,先加人生物素化―抗兔IgG(二抗)孵育,洗涤后,滴加SABC-Cy
内腔可产生高强度的磁场,足以分离磁微粒标记的细胞,无需分离柱提供提供更高强度的磁场。由于磁微粒极小,不会影响靶细胞的流式分析结果,因此不需要去除。除了现有的定型产品外,客户可选择EasySep® PE、FITC或生物素分选系统与自己的PE、FITC或生物素—偶联的单抗相结合,来富集任何所需的细胞。如果您有自己的小鼠IgG1抗任何细胞的抗体, 也可以使用“Do-It-Yourself”试剂盒做成TAC,用来分选您想要的细胞。此外,还可以根据您的特殊需要,专门为您设计定做试剂盒。用于正选小鼠的细胞
下使用未偶联的抗小鼠二抗(最好是驴抗小鼠)执行额外的封闭步骤来减少背景。确保最终抗体浓度不低于 0.1mg/mL。对于某些测定,将室温下或 4 ºC 下过夜的封闭时间增加到 2 小时可能更有效。在这种情况下,可以使用较低浓度的抗体。在普通封闭步骤之后执行额外的封闭步骤,并确保样品前后都经过清洗。 Q:使用NaveniFlex时,两种一抗可以来自同一物种吗? A:不可以 Q:可以检测蛋白质-RNA的相互作用吗? A:理论上可以实现,但没有适用于所有蛋白质-RNA 相互作用的快速解决方案。我们建议使用
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