- ￥1100 - 61500
- 齐源
- 100339
- 国产/进口
- 2025年07月15日
- FC, IHC-F, IP, Activ, Block, WB, ICC
- 仓鼠
- 小鼠
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
CD3ε
- 级别：
CD3ε Antibody
- 目录编号：
CD3ε Antibody
- 克隆性：
单克隆
- 抗原来源：
仓鼠
- 保质期：
CD3ε Antibody
- 抗体英文名：
Ultra-LEAF™ Purified anti-mouse CD3ε Antibody
- 抗体名：
CD3ε Antibody
- 标记物：
CD3ε Antibody
- 宿主：
仓鼠
- 适应物种：
小鼠
- 免疫原：
CD3ε Antibody
- 亚型：
CD3ε Antibody
- 形态：
CD3ε Antibody
- 应用范围：
FC, IHC-F, IP, Activ, Block, WB, ICC
- 保存条件：
2-8℃
- 浓度：
CD3ε Antibody
- 规格：
100 µg/1 mg/5 mg/25 mg/50 mg/100 mg
| 规格： | 100 µg | 产品价格： | ￥1100.0 |
|---|---|---|---|
| 规格： | 1 mg | 产品价格： | ￥2700.0 |
| 规格： | 5 mg | 产品价格： | ￥9800.0 |
| 规格： | 25 mg | 产品价格： | ￥25600.0 |
| 规格： | 50 mg | 产品价格： | ￥42800.0 |
| 规格： | 100 mg | 产品价格： | ￥61500.0 |
Ultra-LEAF™ Purified anti-mouse CD3ε Antibody详细如下：
Description ：CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Igsuperfamily and primarily expressed on T cells, NK-T cells, and at different levels onthymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with theCD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCRsignal transduction, T cell activation, and antigen recognition by binding the peptide/MHCantigen complex.
Verified Reactivity ：Mouse
Antibody Type ：Monoclonal
Host Species ：Armenian Hamster
Immunogen ：H-2Kb -specific mouse cytotoxic T lymphocyte clone BM10-37
Formulation ：0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxinlevel is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation ：The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration ：The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentrationand expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™solution contains no preservative; handle under aseptic conditions.
Application ：FC - Quality tested、IHC-F, IP, Activ, Block, WB, ICC - Reported in the literature, not verified in house
Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cellsin 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated foroptimal performance for each application.
Description ：CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Igsuperfamily and primarily expressed on T cells, NK-T cells, and at different levels onthymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with theCD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCRsignal transduction, T cell activation, and antigen recognition by binding the peptide/MHCantigen complex.
Verified Reactivity ：Mouse
Antibody Type ：Monoclonal
Host Species ：Armenian Hamster
Immunogen ：H-2Kb -specific mouse cytotoxic T lymphocyte clone BM10-37
Formulation ：0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxinlevel is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation ：The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration ：The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentrationand expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™solution contains no preservative; handle under aseptic conditions.
Application ：FC - Quality tested、IHC-F, IP, Activ, Block, WB, ICC - Reported in the literature, not verified in house
Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cellsin 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated foroptimal performance for each application.
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文献和实验该产品被引用文献
Application References(PubMed link indicatesBioLegend citation)：
1. Leo O, et al. 1987. P. Natl. Acad. Sci. USA 84:1374. (IP, Activ, Block)
2. Kruisbeek AM, et al. 1991. In Current Protocols in Immunology. 3.12.1. (Activ)
3. Duke RC, et al. 1995. Current Protocols in Immunology. 3.17.1.
4. Salvadori S, et al. 1994. J. Immunol. 153:5176. (WB)
5. Payer E, et al. 1991. J. Immunol. 146:2536. (IF)
6. Jacobs H, et al. 1994. Eur. J. Immunol. 24:934. (CMCD)
7. Vossen ACTM, et al. 1995. Eur. J. Immunol. 25:1492. (Activ)
8. Henrickson M, et al. 1995. Transplantation 60:828. (Deplete)
9. Kinnaert P, et al. 1996. Transpl. Int. 9:386. (Deplete)
10. Han WR, et al. 1999. Transpl. Immunol. 7:207. (Deplete)
11. Miescher GC, et al. 1989. Immunol. Lett. 23:113. (Block)
12. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Activ)
13. Ko SY, et al. 2005. J. Immunol. 175:3309.
14. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC-F)
15. Tilley SL, et al. 2007. J. Immunol. 178:3208. (IHC-F)
16. Wang W, et al. 2007. J. Immunol. 178:4885. (Activ)
17. Xiao S, et al. 2007. J. Exp. Med. 204:1691.
18. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed.
19. Curtsinger JM, et al.2005. J. Immunol. 175:4392. PubMed
20. Guo Y, et al. 2008. Blood 112:480. PubMed
21. Kenna TJ, et al. 2008. Blood 111:2091.
22. Perchonock CE, et al. 2007. J. Immunol. 179:1768. PubMed
23. Perchonock GE, et al. 2006. Mol. Cell. Biol. 26:6005. PubMed
24. Kanaya T, et al. 2008. Am. J. Physiol. Gastrointest. Liver Physiol. 295:G273. PubMed
25. de Koning BA, et al. 2006. Int. Immunol. 18:941. PubMed
26. Schulteis RD, et al. 2008. Blood 295:G273. PubMed
27. Qi Q, et al. 2009. Blood 114:564. PubMed
28. Helmersson S, et al. 2013. Am J Pathol. 9440:123. Pubmed
29. Wu S, et al. 2014. Clin Vaccine Immunol. 21:156. PubMed
30. Yan J, et al. 2014. Vaccine. 32:2833. PubMed
31. Guiterrez DA, et al. 2014. Diaebetes. 63:3827. PubMed
32. Shi YF, et al. 1991. J Immunol. 146:3340. (Apop)
Product Citations：
1. Dong MB, et al. 2020. Cell. 178(5):1189-1204.e23.. PubMed
2. Jairaman A, et al. 2021. Bio Protoc. 11:e4170. PubMed
3. Duong–Ly KC, et al. 2018. J Cell Sci. 131:17. PubMed
4. Denk D, et al. 2022. Immunity. 55:2059. PubMed
5. Li Q, et al. 2022. Cell Rep. 40:111308. PubMed
6. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
7. Qi Z, et al. 2022. Nat Commun. 13:182. PubMed
8. Gupta SS, et al. 2019. Cell Rep. 29:1862. PubMed
9. Donado CA, et al. 2020. Cell Reports. 31(1):107466. PubMed
10. Wang W, et al. 2020. Cell Rep. 107936:32. PubMed
11. Zhao X, et al. 2021. Int J Oral Sci. 13:31. PubMed
12. Byun JK, et al. 2020. Molecular Cell. 80(4):592-606.e8. PubMed
1. Leo O, et al. 1987. P. Natl. Acad. Sci. USA 84:1374. (IP, Activ, Block)
2. Kruisbeek AM, et al. 1991. In Current Protocols in Immunology. 3.12.1. (Activ)
3. Duke RC, et al. 1995. Current Protocols in Immunology. 3.17.1.
4. Salvadori S, et al. 1994. J. Immunol. 153:5176. (WB)
5. Payer E, et al. 1991. J. Immunol. 146:2536. (IF)
6. Jacobs H, et al. 1994. Eur. J. Immunol. 24:934. (CMCD)
7. Vossen ACTM, et al. 1995. Eur. J. Immunol. 25:1492. (Activ)
8. Henrickson M, et al. 1995. Transplantation 60:828. (Deplete)
9. Kinnaert P, et al. 1996. Transpl. Int. 9:386. (Deplete)
10. Han WR, et al. 1999. Transpl. Immunol. 7:207. (Deplete)
11. Miescher GC, et al. 1989. Immunol. Lett. 23:113. (Block)
12. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Activ)
13. Ko SY, et al. 2005. J. Immunol. 175:3309.
14. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC-F)
15. Tilley SL, et al. 2007. J. Immunol. 178:3208. (IHC-F)
16. Wang W, et al. 2007. J. Immunol. 178:4885. (Activ)
17. Xiao S, et al. 2007. J. Exp. Med. 204:1691.
18. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed.
19. Curtsinger JM, et al.2005. J. Immunol. 175:4392. PubMed
20. Guo Y, et al. 2008. Blood 112:480. PubMed
21. Kenna TJ, et al. 2008. Blood 111:2091.
22. Perchonock CE, et al. 2007. J. Immunol. 179:1768. PubMed
23. Perchonock GE, et al. 2006. Mol. Cell. Biol. 26:6005. PubMed
24. Kanaya T, et al. 2008. Am. J. Physiol. Gastrointest. Liver Physiol. 295:G273. PubMed
25. de Koning BA, et al. 2006. Int. Immunol. 18:941. PubMed
26. Schulteis RD, et al. 2008. Blood 295:G273. PubMed
27. Qi Q, et al. 2009. Blood 114:564. PubMed
28. Helmersson S, et al. 2013. Am J Pathol. 9440:123. Pubmed
29. Wu S, et al. 2014. Clin Vaccine Immunol. 21:156. PubMed
30. Yan J, et al. 2014. Vaccine. 32:2833. PubMed
31. Guiterrez DA, et al. 2014. Diaebetes. 63:3827. PubMed
32. Shi YF, et al. 1991. J Immunol. 146:3340. (Apop)
Product Citations：
1. Dong MB, et al. 2020. Cell. 178(5):1189-1204.e23.. PubMed
2. Jairaman A, et al. 2021. Bio Protoc. 11:e4170. PubMed
3. Duong–Ly KC, et al. 2018. J Cell Sci. 131:17. PubMed
4. Denk D, et al. 2022. Immunity. 55:2059. PubMed
5. Li Q, et al. 2022. Cell Rep. 40:111308. PubMed
6. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
7. Qi Z, et al. 2022. Nat Commun. 13:182. PubMed
8. Gupta SS, et al. 2019. Cell Rep. 29:1862. PubMed
9. Donado CA, et al. 2020. Cell Reports. 31(1):107466. PubMed
10. Wang W, et al. 2020. Cell Rep. 107936:32. PubMed
11. Zhao X, et al. 2021. Int J Oral Sci. 13:31. PubMed
12. Byun JK, et al. 2020. Molecular Cell. 80(4):592-606.e8. PubMed
相关实验
7211011 小鼠淋巴细胞分离液 达优 100339 Ultra-LEAF™ Purified anti-mouse CD3ε Antibody BioLegend 102115 Ultra-LEAF™ Purified anti-mouse CD28 Antibody BioLegend CD3/CD28 Streptamer® 多聚体法： 货号 名称
买。 3、SpinSep® DM-M小鼠专用的淋巴细胞分离液与人用的密度不同，不能混用。 4、可订制的抗体包括：CD2，CD3，CD4，CD5，CD8，CD11b，CD45R，Gr-1，TER119，F4/80，Neutrophil (7-4)，Pan NK (DX5) 5、如果您有自己的大鼠抗小鼠IgG1抗体，并希望用SpinSep® 进行负选，您只需购买SpinSep® 密度粒子和小鼠淋巴细胞分离液。
/Cytoperm液、Perm/Wash液和FACS Lysing Solution，均购自BD Pharmingen。（4）抗体细胞表面标记抗体：抗人CD3-APC，CD8-PercP，CD8-CYC，CD4-CYC。抗小鼠CD4-CYC、CD8PE。细胞内因子抗体：抗人IL-4-PE，IFN-γ-FITC。抗鼠IL-4-PE，IFN-γ-FITC。以上抗体均购自BD Pharmingen。2、实验方法（1）外周血单个核细胞的分离：取肝素抗凝正常人或者Balb/c小鼠外周血1.5ml。在试管内加入淋巴
文献支持
Ultra-LEAF™ Purified 抗小鼠 CD3ε 抗体
￥1100 - 61500
