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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
HDAC2
- 级别:
HDAC2 Antibody
- 目录编号:
HDAC2 Antibody
- 克隆性:
单克隆
- 抗原来源:
小鼠
- 保质期:
HDAC2 Antibody
- 抗体英文名:
Direct-Blot™ HRP anti-HDAC2 Antibody
- 抗体名:
HDAC2 Antibody
- 标记物:
HDAC2 Antibody
- 宿主:
小鼠
- 适应物种:
人、小鼠
- 免疫原:
HDAC2 Antibody
- 亚型:
HDAC2 Antibody
- 形态:
HDAC2 Antibody
- 应用范围:
WB
- 保存条件:
2-8℃
- 浓度:
HDAC2 Antibody
- 规格:
100 µL
Description :HDAC2 is a member of the class I histone deacetylase (HDACs) family, which modulates thechromatin structure by removing acetyl groups from the side chain of lysine residues on the Nterminal region of core histones. HDAC2 lacks DNA binding activity and executes its function byrecruiting transcription factors and forming large transcriptional repressor complexes. Inaddition to histones, HDAC2 can deacetylate transcription factors and modify theirtranscriptional activity. HDAC2 is highly homologous to HDAC1 in regulating cell proliferation,differentiation, and apoptosis. HDAC2 has been shown to negatively regulate synaptic plasticityand memory formation. Elevated activity of HDAC2 has been associated with the loss ofmemory and neuronal degeneration.
Verified Reactivity :Human, Mouse
Antibody Type :Monoclonal
Host Species :Mouse
Immunogen :Human HDAC2 peptide (471-488 a.a.) conjugated to KLH
Formulation :This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation :The antibody was purified by affinity chromatography and conjugated with HRP under optimalconditions.
Concentration :Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)
Storage & Handling :Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected fromprolonged exposure to light.
Application :WB - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by Western blotting. For Western blotting, thesuggested dilution is 1:5000-1:50000. The optimal dilution should be determined by titration foreach individual assay of interest.
25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Westernblots, respectively, at the recommended concentration/dilution.
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文献和实验盐水。 (6)PH7.8饱和硫酸铵溶液及半饱和硫酸铵溶液。 (7)萘氏试剂及聚乙二醇(PEG,MW2000)。 (8)纯化的特异性抗体或抗Ig抗体。 (9)HRP(RZ>3.0)。 (10)Sephadex G-25层析柱(2cm×50cm)。 (11)搅拌器,分光光度计,离心机。 (12)透析袋,大、小烧杯,试管,吸管等。 二、简易过碘酸钠法 本法是以NaIO4先将HRP表面的糖分子氧化成醛基,然后再与Ig上的氨基相结合,所获酶标记抗体的产率高,将近70%的HRP和Ig结合,99%的Ig
氢钠7ml混合。 (4) 0.2M赖氨酸溶液:称赖氨酸29.2mg溶于0.01M PH9.5碳酸缓冲液1ml中。 (5) 0.15M PH7.4 PBS及生理盐水。 (6) PH7.8饱和硫酸铵溶液及半饱和硫酸铵溶液。 (7) 萘氏试剂及聚乙二醇(PEG,MW2000)。 (8) 纯化的特异性抗体或抗Ig抗体。 (9) HRP(RZ>3.0)。 (10) Sephadex G-25层析柱(2cm×50cm)。 (11) 搅拌
人抗狂犬病毒抗体(anti-RV)酶联免疫分析(ELISA)
,再与 HRP 标记的 犬狂犬病毒抗体 结合,形成抗体 - 抗原 - 酶标抗体复合物,经过彻底洗涤后加底物 TMB 显色。 TMB 在 HRP 酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的 抗狂犬病毒抗体 (anti-RV) 呈正相关。用酶标仪在 450nm 波长下测定吸光度( OD 值),通过标准曲线计算样品中 人抗狂犬病毒抗体 (anti-RV) 浓度。 试剂盒组成 : 试剂盒组成











